促炎单核细胞在杜氏肌营养不良症中增加，并被ω-3 脂肪酸抑制：一项双盲、随机、安慰剂对照的初步研究。

Pro-inflammatory monocytes are increased in Duchenne muscular dystrophy and suppressed with omega-3 fatty acids: A double-blind, randomized, placebo-controlled pilot study.

机构信息

Laboratorio de Nutrición Molecular, Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Ciudad de México (CDMX), México.

Departamento de Genética Médica, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México (CDMX), México.

出版信息

Eur J Neurol. 2022 Mar;29(3):855-864. doi: 10.1111/ene.15184. Epub 2021 Nov 26.

DOI:10.1111/ene.15184

PMID:34779542

Abstract

BACKGROUND

Omega-3 long chain polyunsaturated fatty acids (LCPUFA) reduce circulating cytokines produced by monocytes. Nevertheless, whether the omega-3 LCPUFA regulate the monocytes and their cytokines in Duchenne muscular dystrophy (DMD) is unknown. The aim of this study was to evaluate whether circulating pro-inflammatory monocytes are increased and whether omega-3 LCPUFA selectively suppress these monocytes and their cytokines in patients with DMD.

METHODS

This was a double-blind, randomized, placebo-controlled pilot study carried out in patients with DMD supplemented with omega-3 LCPUFA (n = 6) or sunflower oils (placebo, n = 6) for 6 months. Monocytes and their cytokines were measured at baseline and after 1, 2, 3, and 6 months of supplementation.

RESULTS

The anti-inflammatory monocytes (median, [95% CI]) are increased at month 3 (-0.46 [-13.5-9.5] vs. 8.4 [5.5-12.5], p = 0.05) in the omega-3 LCPUFA group compared with the placebo group. The pro-inflammatory monocytes (-5.7 [-63.8-114.1] vs. -51.9 [-91.2 to -25.4], p = 0.026 and -16.4 [-50.8-50.6] vs. -57.9 [-86.9 to -18.5], p = 0.045 at months 3 and 6, respectively) and their cytokine interleukin 6 (-11.9 [-93.5-148.9] vs. -64.7 [-77.8 to -42.6], p = 0.019 at month 6) decreased in the omega-3 LCPUFA group compared with the placebo group. Pro-inflammatory monocytes decreased and anti-inflammatory monocytes were augmented (p < 0.05) during the 6 months of supplementation with omega-3 LCPUFA.

CONCLUSIONS

This pilot study suggests that supplementation with omega-3 LCPUFA could have a selective reductive effect on pro-inflammatory monocytes and their cytokines in patients with DMD. These findings also support the performance of studies in a significant population to explore the role of omega-3 LCPUFA on monocyte populations and their cytokines in patients with DMD. This research was registered at clinicaltrials.gov (NCT018264229).

摘要

背景

ω-3 长链多不饱和脂肪酸（LCPUFA）可减少单核细胞产生的循环细胞因子。然而，ω-3 LCPUFA 是否调节 Duchenne 肌营养不良症（DMD）中的单核细胞及其细胞因子尚不清楚。本研究旨在评估循环促炎单核细胞是否增加，以及 ω-3 LCPUFA 是否选择性地抑制 DMD 患者的这些单核细胞及其细胞因子。

方法

这是一项双盲、随机、安慰剂对照的初步研究，对补充 ω-3 LCPUFA（n=6）或葵花籽油（安慰剂，n=6）的 DMD 患者进行了 6 个月的治疗。在基线时以及补充后 1、2、3 和 6 个月时测量单核细胞及其细胞因子。

结果

与安慰剂组相比，ω-3 LCPUFA 组在第 3 个月时抗炎单核细胞（中位数，[95%CI]）增加（-0.46[-13.5-9.5] 与 8.4[5.5-12.5]，p=0.05）。促炎单核细胞（-5.7[-63.8-114.1] 与-51.9[-91.2 至-25.4]，p=0.026 和-16.4[-50.8-50.6] 与-57.9[-86.9 至-18.5]，p=0.045，分别在第 3 个月和第 6 个月）及其细胞因子白细胞介素 6（-11.9[-93.5-148.9] 与-64.7[-77.8 至-42.6]，p=0.019，在第 6 个月）在 ω-3 LCPUFA 组中减少。与安慰剂组相比，ω-3 LCPUFA 补充 6 个月后，促炎单核细胞减少，抗炎单核细胞增加（p<0.05）。

结论

这项初步研究表明，ω-3 LCPUFA 补充可能对 DMD 患者的促炎单核细胞及其细胞因子具有选择性的减少作用。这些发现还支持在更大的人群中进行研究，以探讨 ω-3 LCPUFA 对 DMD 患者单核细胞群及其细胞因子的作用。本研究已在 clinicaltrials.gov 注册（NCT018264229）。