Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China.
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005091.
Two novel bacterial strains, designated as DN00404 and DN04309, were isolated from aquaculture water and characterized by using a polyphasic taxonomic approach. Cells of strains DN00404 and DN04309 were Gram-stain-negative, aerobic, non-motile, oxidase-positive and catalase-positive. Cells of DN00404 were short rod-shaped and those of DN04309 were long rod-shaped. Strain DN00404 was found to grow at 15-37 °C (optimum, 25-30 °C), at pH 6.0-11.0 (optimum, pH 7.5) and in 0-2.0 % (w/v) NaCl (optimum, 1.0 %). Strain DN04309 was found to grow at 15-45 °C (optimum, 20-37 °C), at pH 5.5-11.0 (optimum, 7.5) and in 0-4.0 % (w/v) NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene and genome sequences revealed that the two strains belonged to the genus and were distinct from all known species of this genus. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains and between each of the two strains and related type strains of this genus were well below the recognized thresholds of 95.0-96.0 % ANI and 70.0 % dDDH for species delineation. The genomic DNA G+C contents of strains DN00404 and DN04309 were 41.6 and 36.0 mol%, respectively. The respiratory quinone in both strains was identified as MK-7, and their major fatty acids were iso-C and summed feature 3 (C 6 and/or C 7), which were similar to those of other species of this genus. The two major fatty acids C and iso-C 3-OH were also found in strain DN00404. Based on genotypic and phenotypic characteristics, two novel species of the genus are proposed: sp. nov. with DN00404 (=GDMCC 1.1865=KACC 21924) as the type strain and sp. nov. with DN04309 (=GDMCC 1.1984=KCTC 82348) as the type strain.
从养殖水中分离到两株新的细菌菌株，分别命名为 DN00404 和 DN04309，并采用多相分类方法进行了表征。菌株 DN00404 和 DN04309 的细胞革兰氏染色阴性、需氧、不运动、氧化酶阳性和过氧化氢酶阳性。DN00404 菌株的细胞呈短杆状，DN04309 菌株的细胞呈长杆状。菌株 DN00404 被发现可在 15-37°C（最佳，25-30°C）、pH6.0-11.0（最佳，pH7.5）和 0-2.0%（w/v）NaCl（最佳，1.0%）下生长。菌株 DN04309 被发现可在 15-45°C（最佳，20-37°C）、pH5.5-11.0（最佳，7.5）和 0-4.0%（w/v）NaCl（最佳，0.5%）下生长。基于 16S rRNA 基因和基因组序列的系统发育分析表明，这两株菌属于 属，与该属的所有已知种均不同。两株菌之间以及与该属的每个相关模式种之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值均远低于种间 95.0-96.0%ANI 和 70.0% dDDH 的公认阈值。菌株 DN00404 和 DN04309 的基因组 DNA G+C 含量分别为 41.6 和 36.0 mol%。两株菌的呼吸醌均鉴定为 MK-7，其主要脂肪酸为 iso-C 和总和特征 3（C6 和/或 C7），与该属的其他种相似。在菌株 DN00404 中还发现了两种主要脂肪酸 C 和 iso-C 3-OH。基于基因型和表型特征，提出了该属的两个新种： sp. nov.，以 DN00404（=GDMCC 1.1865=KACC 21924）作为模式株； sp. nov.，以 DN04309（=GDMCC 1.1984=KCTC 82348）作为模式株。
