National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China.
Int J Syst Evol Microbiol. 2024 Aug;74(8). doi: 10.1099/ijsem.0.006488.
Four strains, designated dk4302, dk4209, xlx-73, and xlx-183, were isolated from Tibetan gazelle and red swamp crawfish collected from the Qinghai-Tibet Plateau and Jiangxi Province, PR China. The strains were Gram-stain-negative, aerobic, rod-shaped, non-motile, mucoid, and yellow-pigmented. Strains dk4302 and dk4209 grew at 10-40 °C and pH 6.0-9.0, while strains xlx-73/xlx-183 grew at 15-40 °C and pH 6.0-10.0. Both strains exhibited growth in the presence of up to 3.5 % (w/v) NaCl. Phylogenetic and phylogenomic analyses based on the 16S rRNA gene sequences and 652 core genes, respectively, revealed that the four strains formed two distinct clusters in the genus . Strains dk4302 and dk4209 formed a distinct clade with XH4 and D1. The most closely related strains to xlx-73 and xlx-183 were M-SX103. The DNA G+C contents were 38.9 and 39.8 mol%. The digital DNA-DNA hybridization (dDDH) values between dk4302 and D1 and XH4 were 19.2 and 21.8 % (19.0 and 21.6 % for strain dk4209), respectively. The corresponding average nucleotide identity (ANI) values were 74.3 and 78.1 % (74.4 and 78.3 % for strain dk4209), respectively. The dDDH values between xlx-73 (xlx-183) and M-SX103 was 24.6 % (25.7 %). The corresponding ANI value was 85.7 % (85.5 % for strain xlx-183). The major fatty acid and respiratory quinone of dk4302 and xlx-73 were iso-C and MK7. The polar lipids identified in all of the novel strains were phosphatidylethanolamine, phosphoglycolipids, aminophospholipids, and phospholipids. A total of 61/190 (32.1 %) and 82/190 (43.2 %) carbon substrates were metabolized by strains dk4302 and xlx-73 in the Biolog MicroPlates, respectively. Based on the results from this polyphasic taxonomic study, two novel species in the genus are proposed, namely sp. nov. (type strain dk4302=CGMCC 1.16795=JCM 33600) and sp. nov. (type strain xlx-73=GDMCC 1.1712=JCM 33886).
从青藏高原和中国江西省采集的藏羚羊和红沼泽小龙虾中分离到了 4 株菌，分别命名为 dk4302、dk4209、xlx-73 和 xlx-183。这些菌株革兰氏阴性、需氧、杆状、非运动、黏液状和黄色色素。菌株 dk4302 和 dk4209 在 10-40°C 和 pH 6.0-9.0 下生长，而菌株 xlx-73/xlx-183 在 15-40°C 和 pH 6.0-10.0 下生长。两种菌株在高达 3.5%（w/v）NaCl 的存在下都能生长。基于 16S rRNA 基因序列和 652 个核心基因的系统发育和基因组分析分别表明，这 4 株菌在属内形成了两个不同的聚类。菌株 dk4302 和 dk4209 与 XH4 和 D1 形成了一个独特的分支。与 xlx-73 和 xlx-183 最密切相关的菌株是 M-SX103。DNA G+C 含量分别为 38.9 和 39.8mol%。dk4302 与 D1 和 XH4 的 DNA-DNA 杂交（dDDH）值分别为 19.2%和 21.8%（菌株 dk4209 为 19.0%和 21.6%）。相应的平均核苷酸同一性（ANI）值分别为 74.3%和 78.1%（菌株 dk4209 为 74.4%和 78.3%）。xlx-73（xlx-183）与 M-SX103 的 dDDH 值为 24.6%（菌株 xlx-183 为 25.7%）。相应的 ANI 值为 85.7%（菌株 xlx-183 为 85.5%）。dk4302 和 xlx-73 的主要脂肪酸和呼吸醌为 iso-C 和 MK7。所有新菌株中鉴定的极性脂均为磷脂酰乙醇胺、磷酸甘油脂、氨基磷脂和磷脂。菌株 dk4302 和 xlx-73 在 Biolog MicroPlates 中分别代谢了 61/190（32.1%）和 82/190（43.2%）的碳底物。基于多相分类学研究的结果，提出了属中的两个新种，即. sp. nov.（dk4302 型菌株=CGMCC 1.16795=JCM 33600）和. sp. nov.（xlx-73 型菌株=GDMCC 1.1712=JCM 33886）。
