College of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an, 710054, Shaanxi, China.
College of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an, 710054, Shaanxi, China.
Environ Pollut. 2022 Jan 15;293:118486. doi: 10.1016/j.envpol.2021.118486. Epub 2021 Nov 12.
A previous study has revealed that the parameter k∙EC, characterizing the shape of concentration-response curves (CRCs), could predict the combined toxicity of binary mixtures. This study further explored the predictability of multi-component mixtures. Eleven component mixtures were designed using the uniform design ray, and the acute toxicity of the eleven environmental pollutants and their mixtures to Vibrio fischeri was determined using microplate toxicity analysis. We used independent action (IA) and the effect residual ratio (ERR) models to evaluate the combined toxicity of multi-component mixtures and ascertain the functional relationship between σ, a parameter characterizing the CRC morphological difference of multi-component mixtures, and combined toxicity. The variance σ of each component characteristic parameter of multi-component mixtures gradually increased in the concentration range, and the relationship between σ and ERR was consistent with the exponential function. The literature verification showed that this rule is generally applicable to the acute toxicity of multi-component mixtures to luminescent bacteria. The exponential function showed the variation rule of the joint action of multi-component mixtures. In the present study, the joint toxicity of multi-component mixtures can be predicted from single toxicity and small amount of multiple toxicity, circumventing complex multi-component toxicity experiments.
先前的研究表明，能够描述浓度-反应曲线（CRC）形状的参数 k∙EC，可以预测二元混合物的联合毒性。本研究进一步探讨了多组分混合物的可预测性。利用均匀设计射线设计了 11 种成分混合物，并采用微孔板毒性分析法测定了 11 种环境污染物及其混合物对发光菌的急性毒性。采用独立作用（IA）和效应残差（ERR）模型评价多组分混合物的联合毒性，确定参数 σ与联合毒性之间的功能关系，该参数用于描述多组分混合物 CRC 形态差异。多组分混合物各成分特征参数的方差 σ在浓度范围内逐渐增大，且 σ 与 ERR 之间的关系符合指数函数。文献验证表明，该规律一般适用于多组分混合物对发光菌的急性毒性。指数函数反映了多组分混合物联合作用的变化规律。本研究从单一毒性和少量多种毒性出发，预测多组分混合物的联合毒性，避免了复杂的多组分毒性实验。
