College of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an 710054, Shaanxi, China.
College of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an 710054, Shaanxi, China.
Environ Toxicol Pharmacol. 2022 Aug;94:103917. doi: 10.1016/j.etap.2022.103917. Epub 2022 Jun 30.
A previous acute toxicity study of binary mixtures showed that the combined toxicity can be predicted with the parameter k∙EC. To systematically investigate the ability of k∙EC to predict the chronic combined toxicity of binary mixtures, the toxicity of six contaminants and five binary mixtures was determined by long-term microplate toxicity analysis (L-MTA) using Aliivibrio fischeri as the test organism. The independent action model (IA) and the relative model deviation ratio (rMDR) were employed to determine the relationship between the Δ(k∙EC)% and rMDR. The results showed that these two factors conformed to the exponential function in long-term toxicity. Owing to the time-dependence of toxicity, the mixture type of chronic toxicity changes to the relative type of acute toxicity. If the acute toxicity of binary mixtures changes their mode of joint action throughout the concentration range, the chronic toxicity will also change their mode of joint action, and vice versa. This study clarified the change rules of the joint action of binary mixtures in acute and chronic toxicity which can promote research on chronic toxicity of binary mixtures.
先前的二元混合物急性毒性研究表明，联合毒性可以用参数 k·EC 预测。为了系统研究 k·EC 预测二元混合物慢性联合毒性的能力，使用发光菌 Aliivibrio fischeri 作为测试生物，通过长期微孔板毒性分析（L-MTA）确定了六种污染物和五种二元混合物的毒性。采用独立作用模型（IA）和相对模型偏差比（rMDR）来确定 Δ(k·EC)%和 rMDR 之间的关系。结果表明，这两个因素在长期毒性中符合指数函数。由于毒性的时间依赖性，慢性毒性的混合物类型转变为相对的急性毒性类型。如果二元混合物的急性毒性在整个浓度范围内改变其联合作用模式，慢性毒性也将改变其联合作用模式，反之亦然。本研究阐明了二元混合物在急性和慢性毒性中的联合作用变化规律，这将促进二元混合物慢性毒性的研究。
