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牙本质微结构的发育由相邻上皮的类型控制。

The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium.

机构信息

Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Advanced Instrumentation and Methods for Materials Characterization, CEITEC Brno University of Technology, Brno, Czech Republic.

出版信息

J Bone Miner Res. 2022 Feb;37(2):323-339. doi: 10.1002/jbmr.4471. Epub 2021 Dec 12.

DOI:10.1002/jbmr.4471
PMID:34783080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9300090/
Abstract

Considerable amount of research has been focused on dentin mineralization, odontoblast differentiation, and their application in dental tissue engineering. However, very little is known about the differential role of functionally and spatially distinct types of dental epithelium during odontoblast development. Here we show morphological and functional differences in dentin located in the crown and roots of mouse molar and analogous parts of continuously growing incisors. Using a reporter (DSPP-cerulean/DMP1-cherry) mouse strain and mice with ectopic enamel (Spry2 ;Spry4 ), we show that the different microstructure of dentin is initiated in the very beginning of dentin matrix production and is maintained throughout the whole duration of dentin growth. This phenomenon is regulated by the different inductive role of the adjacent epithelium. Thus, based on the type of interacting epithelium, we introduce more generalized terms for two distinct types of dentins: cementum versus enamel-facing dentin. In the odontoblasts, which produce enamel-facing dentin, we identified uniquely expressed genes (Dkk1, Wisp1, and Sall1) that were either absent or downregulated in odontoblasts, which form cementum-facing dentin. This suggests the potential role of Wnt signalling on the dentin structure patterning. Finally, we show the distribution of calcium and magnesium composition in the two developmentally different types of dentins by utilizing spatial element composition analysis (LIBS). Therefore, variations in dentin inner structure and element composition are the outcome of different developmental history initiated from the very beginning of tooth development. Taken together, our results elucidate the different effects of dental epithelium, during crown and root formation on adjacent odontoblasts and the possible role of Wnt signalling which together results in formation of dentin of different quality. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

摘要

大量的研究集中在牙本质矿化、成牙本质细胞分化及其在牙组织工程中的应用。然而，对于在成牙本质细胞发育过程中功能和空间上不同类型的牙上皮的差异作用知之甚少。在这里，我们展示了小鼠磨牙的牙冠和牙根以及不断生长的切牙的类似部位的牙本质的形态和功能差异。使用报告基因（DSPP- cerulean / DMP1-cherry ）小鼠品系和具有异位釉质的小鼠（Spry2 ； Spry4 ），我们表明牙本质的不同微观结构是在牙本质基质产生的早期开始的，并在牙本质生长的整个过程中保持不变。这种现象受相邻上皮的不同诱导作用的调节。因此，基于相互作用的上皮类型，我们为两种不同类型的牙本质引入了更通用的术语：牙骨质与牙釉质面向的牙本质。在产生牙釉质面向牙本质的成牙本质细胞中，我们鉴定了独特表达的基因（Dkk1 、 Wisp1 和 Sall1 ），这些基因在形成牙骨质面向牙本质的成牙本质细胞中要么不存在，要么下调。这表明 Wnt 信号在牙本质结构模式形成中的潜在作用。最后，我们通过利用空间元素组成分析（LIBS）来显示两种发育上不同类型牙本质的钙和镁组成的分布。因此，牙本质内部结构和元素组成的变化是从牙齿发育早期开始的不同发育历史的结果。总之，我们的结果阐明了牙上皮在牙冠和牙根形成过程中对相邻成牙本质细胞的不同影响，以及 Wnt 信号的可能作用，这些共同导致了不同质量的牙本质的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/a33b2ebac70a/JBMR-37-323-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/6fbd9ae25ec2/JBMR-37-323-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/a6e3053122ff/JBMR-37-323-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/6af147e757f7/JBMR-37-323-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/10a8a22e963b/JBMR-37-323-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/57562c6a8d16/JBMR-37-323-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/4cc1c42cf930/JBMR-37-323-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/32143366a778/JBMR-37-323-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/85c975bcc026/JBMR-37-323-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/a33b2ebac70a/JBMR-37-323-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/6fbd9ae25ec2/JBMR-37-323-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/a6e3053122ff/JBMR-37-323-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/6af147e757f7/JBMR-37-323-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/10a8a22e963b/JBMR-37-323-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/57562c6a8d16/JBMR-37-323-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/4cc1c42cf930/JBMR-37-323-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/32143366a778/JBMR-37-323-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/85c975bcc026/JBMR-37-323-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea0/9300090/a33b2ebac70a/JBMR-37-323-g003.jpg

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引用本文的文献

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Spatiotemporal cell landscape of human embryonic tooth development.

Cell Prolif. 2024-9

[2]
The role of vitamin D receptor in predentin mineralization and dental repair after injury.

Cell Tissue Res. 2024-6

[3]
Cellular Signaling for Dental Physiological Functions.

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[4]
The investigation of WNT6 and WNT10A single nucleotide polymorphisms as potential biomarkers for dental pulp calcification in orthodontic patients.

PLoS One. 2023

[5]
Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development.

Sci Adv. 2023-8-2

[6]
Expression Levels of WNT Signaling Pathway Genes During Early Tooth Development.

Organogenesis. 2023-12-31

[7]
Plasticity of Dental Cell Types in Development, Regeneration, and Evolution.

J Dent Res. 2023-6

[8]
Piezo1-pannexin-1-P2X axis in odontoblasts and neurons mediates sensory transduction in dentinal sensitivity.

Front Physiol. 2022-12-14

本文引用的文献

[1]
Pivotal Role of Tenascin-W (-N) in Postnatal Incisor Growth and Periodontal Ligament Remodeling.

Front Immunol. 2021-1-22

[2]
Methodology and applications of elemental mapping by laser induced breakdown spectroscopy.

Anal Chim Acta. 2021-2-22

[3]
Odontoblast processes of the mouse incisor are plates oriented in the direction of growth.

Anat Rec (Hoboken). 2021-8

[4]
SALL1 regulates commitment of odontoblast lineages by interacting with RUNX2 to remodel open chromatin regions.

Stem Cells. 2021-2

[5]
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.

Nat Commun. 2020-9-23

[6]
Runx2 Regulates Mouse Tooth Root Development Via Activation of WNT Inhibitor NOTUM.

J Bone Miner Res. 2020-11

[7]
Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice.

Genesis. 2019-10

[8]
Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process.

Int J Biol Sci. 2018-4-30

[9]
TRPM7 Mediates Mechanosensitivity in Adult Rat Odontoblasts.

J Dent Res. 2018-2-28

[10]
A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

Nat Commun. 2018-1-25

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