School of Dentistry, University of São Paulo (FORP-USP), Ribeirão Preto, Brazil.
Department of Orthodontics, University of Regensburg, Regensburg, Germany.
PLoS One. 2023 Aug 11;18(8):e0288782. doi: 10.1371/journal.pone.0288782. eCollection 2023.
The aim of this study is to evaluate if single nucleotide polymorphisms (SNPs) in WNT6 and WNT10A are associated with the risk of dental pulp calcification in orthodontic patients. This cross-sectional study followed the "Strengthening the Reporting of Genetic Association Studies" (STREGA) guidelines. Panoramic radiographs (pre- and post-orthodontic treatment) and genomic DNA from 132 orthodontic patients were studied. Dental pulp calcification (pulp stones and/or pulp space narrowing) was recorded in upper and lower first molars. The SNPs in WNT6 and WNT10A (rs7349332, rs3806557, rs10177996, and rs6754599) were assessed through genotyping analysis using DNA extracted from buccal epithelial cells. The association between pulp calcification and SNPs were analyzed using allelic and genotypic distributions and haplotype frequencies (p<0.05). Prevalence of dental pulp calcification was 42.4% in the 490 studied molars. In the genotypic analysis, the SNPs in WNT10A showed a statistically significant value for molar calcification (p = 0.027 for rs1017799), upper molar calcification (p = 0.040 for rs1017799) (recessive model), and molar calcification (p = 0.046 for rs3806557) (recessive model). In the allelic distribution, the allele C of the SNP rs10177996 in WNT10A was associated with molar calcifications (p = 0.042) and with upper first molar calcification (p = 0.035). Nine combinations of haplotypes showed statistically significant value (p<0.05). The findings of this study indicates that SNPs in WNT10A and WNT6 are associated with dental pulp calcification in molars after orthodontic treatment and may be considered as biomarkers for dental pulp calcification.
本研究旨在评估 WNT6 和 WNT10A 中的单核苷酸多态性（SNPs）是否与正畸患者牙髓钙化的风险相关。这项横断面研究遵循了“遗传关联研究报告强化标准”（STREGA）指南。研究了 132 名正畸患者的全景片（正畸治疗前后）和基因组 DNA。记录了上颌和下颌第一磨牙的牙髓钙化（牙髓结石和/或牙髓腔变窄）。使用从颊上皮细胞提取的 DNA 通过基因分型分析评估了 WNT6 和 WNT10A 中的 SNPs（rs7349332、rs3806557、rs10177996 和 rs6754599）。使用等位基因和基因型分布以及单倍型频率（p<0.05）分析了牙髓钙化与 SNPs 的关系。在研究的 490 颗磨牙中，牙髓钙化的患病率为 42.4%。在基因型分析中，WNT10A 中的 SNPs 显示出磨牙钙化的统计学显著值（rs1017799 的 p = 0.027）、上颌磨牙钙化（rs1017799 的 p = 0.040）（隐性模型）和磨牙钙化（rs3806557 的 p = 0.046）（隐性模型）。在等位基因分布中，WNT10A 中 SNP rs10177996 的 C 等位基因与磨牙钙化（p = 0.042）和上颌第一磨牙钙化（p = 0.035）相关。9 种单倍型组合显示出统计学显著值（p<0.05）。本研究的结果表明，WNT10A 和 WNT6 中的 SNPs 与正畸治疗后磨牙的牙髓钙化相关，可作为牙髓钙化的生物标志物。
