Pearl Wrenit Gem, Perevedentseva Elena V, Karmenyan Artashes V, Khanadeev Vitaly A, Wu Sheng-Yun, Ma Yuan-Ron, Khlebtsov Nikolai G, Cheng Chia-Liang
Department of Physics, National Dong Hwa University, Hualien, Taiwan.
P. N. Lebedev Physics Institute of Russian Academy of Sciences, Moscow, Russia.
J Biophotonics. 2022 Mar;15(3):e202100264. doi: 10.1002/jbio.202100264. Epub 2021 Dec 10.
Gold nanostar (AuNSt) has gained great attention in bioimaging and cancer therapy due to their tunable surface plasmon resonance across the visible-near infrared range. Photothermal treatment and imaging capabilities including fluorescence lifetime imaging at two-photon excitation (TP-FLIM) and dark-field microscopic imaging are considered in this work. Two types of AuNSts having plasmon absorption peaks centred at 600 and 750 nm wavelength were synthesized and studied. Both NSts exhibited low cytotoxicity on A549 human lung carcinoma cells. A strong emission at two-photon excitation was observed for both NSts, well-distinguishable from lifetimes of bio-object autofluorescence. High efficiency in raising the temperature in the NSts environment with the irradiation of near infrared, AuNSts triggered photothermal effect. The decreased cell viability of A549 observed via MTT test and the cell membrane damaging was demonstrated with trypan blue staining. These results suggest AuNSts can be agents with tunable plasmonic properties for imaging and photothermal therapy.
金纳米星（AuNSt）因其在可见光-近红外范围内可调谐的表面等离子体共振，在生物成像和癌症治疗方面备受关注。本研究考虑了光热处理和成像能力，包括双光子激发荧光寿命成像（TP-FLIM）和暗场显微成像。合成并研究了两种等离子体吸收峰分别位于600和750 nm波长的AuNSt。两种纳米星对A549人肺癌细胞均表现出低细胞毒性。两种纳米星在双光子激发下均观察到强发射，与生物物体自发荧光的寿命有明显区别。在近红外光照射下，AuNSt能高效提高其周围环境温度，引发光热效应。通过MTT试验观察到A549细胞活力下降，台盼蓝染色证明细胞膜受到损伤。这些结果表明，AuNSt可作为具有可调谐等离子体特性的成像和光热治疗剂。
