IEEE Trans Nanobioscience. 2022 Oct;21(4):520-528. doi: 10.1109/TNB.2021.3128407. Epub 2022 Sep 26.
In the present study, biocompatible nanofibers containing aqueous extracts from Muscari neglectum (M. neglectum) plants (produced nanofiber) were prepared and their antifungal and cytotoxicity effects were investigated. For this purpose, the extracts obtained from flowers, stem leaves, and fresh onion from M. neglectum were lyophilized at various concentrations. Produced nanofibers were prepared using electrospinning techniques. During the electrospinning process, two auxiliary natural polymers including gelatin and chitosan were used. After synthesis, the physicochemical properties of the nanofibers were confirmed by Scanning Electron Microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray energy diffraction spectroscopy (EDS or EDX), and Differential Scanning Calorimetry (DSC). The electrospun produced nanofibers have continuous and uniform structures. The cytotoxicity assay of these electrospun nanofibers were done on Human dermal fibroblast cell (HDF) and HUVEC cell (Human Umbilical Endothelial Cells) lines and results showed that nanofiber doesn't have any toxicity to normal cell lines. For anti-fungal activity tests, the appropriate amounts of nanofibers containing M. neglectum were placed in media with five different fungal species utilizing two methods: disc diffusion and well diffusion. In vitro results showed that all electrospun nanofibers containing M. neglectum had strong antifungal activity against Candida albicans, Glabrata, Parapacillus, Guillermoides, Crocus fungi species. Our findings also showed that nanofibers containing 86.88% polyvinyl alcohol/ gelatin/ chitosan/ M. neglectum root extract (produced nanofibers) were had better swelling and physicochemical properties and stronger antifungal activity than others (fiber formed with plant root). In a nutshell, natural nanofibers can be used as a beneficial drug delivery system.
在本研究中，制备了含有珠芽百合（Muscari neglectum）植物水提物的生物相容性纳米纤维（制备的纳米纤维），并研究了其抗真菌和细胞毒性作用。为此，将从珠芽百合的花、茎叶和新鲜洋葱中提取的提取物在不同浓度下冻干。使用静电纺丝技术制备制备的纳米纤维。在静电纺丝过程中，使用了两种辅助天然聚合物，即明胶和壳聚糖。合成后，通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线能量衍射光谱（EDS 或 EDX）以及差示扫描量热法（DSC）确认纳米纤维的物理化学性质。电纺制备的纳米纤维具有连续和均匀的结构。对这些电纺纳米纤维的细胞毒性进行了测试，分别在人真皮成纤维细胞（HDF）和人脐静脉内皮细胞（HUVEC）系上进行，结果表明纳米纤维对正常细胞系没有任何毒性。为了进行抗真菌活性测试，将适量的含有珠芽百合的纳米纤维放置在含有五种不同真菌的培养基中，采用两种方法：圆盘扩散法和孔扩散法。体外结果表明，所有含有珠芽百合的电纺纳米纤维对白色念珠菌、光滑念珠菌、近平滑念珠菌、热带念珠菌和藏红花菌都具有很强的抗真菌活性。我们的研究结果还表明，含有 86.88%聚乙烯醇/明胶/壳聚糖/珠芽百合根提取物（制备的纳米纤维）的纳米纤维具有更好的溶胀和物理化学性质以及更强的抗真菌活性，优于其他纤维（由植物根形成的纤维）。总之，天然纳米纤维可用作有益的药物传递系统。
