Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Department of Medical Services and Techniques, Şuhut Vocational School of Health Services, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Microsc Res Tech. 2023 Oct;86(10):1309-1321. doi: 10.1002/jemt.24315. Epub 2023 Mar 17.
The present study investigated that chitosan production of Rhizopus oryzae NRRL 1526 and Aspergillus niger ATCC 16404. Fungal chitosans were characterized by scanning electron microscopy (SEM)-energy dispersive X-ray analysis, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter and deacetylation degrees of fungal chitosans were determined. The percentage yield of Ro-chitosan and An-chitosan were determined as 18.6% and 12.5%, respectively. According to percentage of chitosan yield and the results of the characterization studies, chitosan that obtained from Rhizopus oryzae NRRL 1526 was selected for subsequent studies. Cytotoxicity of chitosan obtained from Rhizopus oryzae NRRL 1526 was determined by MTT assay on human dermal fibroblast cell line. Acording to results of the cytotoxicity test fungal chitosan was nontoxic on cells. The high cell viability was observed 375 μg/mL concentration at 24th, 48th h periods and at the 187.5 μg/ml 72nd h periods on cells. The fungal chitosan obtained from Rhizopus oryzae NRRL 1526 was used to fabrication of electrospun nanofibers. Fungal chitosan based polymer solutions were prepared by adding different substances and different electrostatic spinning parameters were used to obtain most suitable nanofiber structure. Characterization studies of nanofibers were carried out by SEM, FTIR and X-ray diffraction. The most suitable nanofiber structure was determined as F4 formula. The nanofiber structure was evaluated to be thin, bead-free, uniform, flexible and easily remove from surface and taking the shape of the area. After the characterization analysis of fungal chitosan it was determined that the chitosan, which obtained from Rhizopus oryzae NRRL 1526 is actually chitosan polymer and this polymer is usable for pharmaceutical areas and biotechnological applications. The electrospun nanofiber that blends fungal chitosan and PCL polymers were fabricated successfully and that it can be used as fabrication wound dressing models. RESEARCH HIGHLIGHTS: Extraction of chitosan from Rhizopus oryzae NRRL 1526 and Aspergillus niger ATCC 16404 and characterization scanning electron microscopy-energy dispersive X-ray analysis, Fourier transform infrared spectroscopy, differential scanning calorimeter. Fabrication and characterization of the fungal chitosan/PCL electrospun nanofibers.
本研究考察了米根霉 NRRL 1526 和黑曲霉 ATCC 16404 的壳聚糖生产。通过扫描电子显微镜（SEM）-能量色散 X 射线分析、傅里叶变换红外光谱（FTIR）、差示扫描量热法对真菌壳聚糖进行了表征，并测定了真菌壳聚糖的脱乙酰度。Ro-壳聚糖和 An-壳聚糖的得率分别为 18.6%和 12.5%。根据壳聚糖的产率百分比和表征研究的结果，选择了来自米根霉 NRRL 1526 的壳聚糖进行后续研究。通过对人真皮成纤维细胞系进行 MTT 测定，测定了来自米根霉 NRRL 1526 的壳聚糖的细胞毒性。根据细胞毒性试验的结果，真菌壳聚糖对细胞无毒。在 24 小时、48 小时和 72 小时的 187.5μg/ml 细胞培养物中，观察到 375μg/ml 浓度的高细胞活力。从米根霉 NRRL 1526 获得的真菌壳聚糖用于制备电纺纳米纤维。通过添加不同的物质来制备真菌壳聚糖基聚合物溶液，并使用不同的静电纺丝参数来获得最适合的纳米纤维结构。通过 SEM、FTIR 和 X 射线衍射对纳米纤维进行了表征研究。最适合的纳米纤维结构确定为 F4 配方。纳米纤维结构被评估为薄、无珠、均匀、灵活且易于从表面去除，并采用该区域的形状。对真菌壳聚糖进行表征分析后，确定从米根霉 NRRL 1526 获得的壳聚糖实际上是壳聚糖聚合物，该聚合物可用于制药领域和生物技术应用。成功制备了混合真菌壳聚糖和 PCL 聚合物的电纺纳米纤维，并可将其用作制造伤口敷料模型。研究亮点：从米根霉 NRRL 1526 和黑曲霉 ATCC 16404 中提取壳聚糖，并通过扫描电子显微镜-能量色散 X 射线分析、傅里叶变换红外光谱、差示扫描量热法进行表征。真菌壳聚糖/PCL 电纺纳米纤维的制备和表征。
