Fathollahipour Shahrzad, Abouei Mehrizi Ali, Ghaee Azadeh, Koosha Mojtaba
Faculty of New Science and Technologies, Department of Life Science Engineering, University of Tehran, Tehran, Iran.
Department of Textile and Polymer Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.
J Biomed Mater Res A. 2015 Dec;103(12):3852-62. doi: 10.1002/jbm.a.35529. Epub 2015 Jul 14.
Nanofibrous core-sheath nanocomposite dual drug delivery system based on poly(vinyl alcohol) (PVA)/chitosan/lidocaine hydrochloride loaded with gelatin nanoparticles were successfully prepared by the electrospinning method. Gelatin nanoparticles were prepared by nanoprecipitation and were then loaded with erythromycin antibiotic agent with the average particle size of ∼175 nm. The morphology of gelatin nanoparticles observed by field emission scanning electron microscopy (FE-SEM) was shown to be optimal at the concentration of 1.25 wt % of gelatin in aqueous phase by addition of 20 µL of glutaraldehyde 5% as the crosslinking agent. The nanoparticles were also characterized by dynamic light scattering, zeta potential measurement, and Fourier transform infrared spectroscopy (FTIR). The best bead free morphology for the PVA/chitosan nanofibrous mats were obtained at the solution weight ratio of 96/4. The nanofibrous mats were analyzed by swelling studies, FTIR and antibacterial tests. In vitro dual release profile of the core-sheath nanofibers was also studied within 72 h and showed the release efficiency equal to 84.69 and 75.13% for lidocaine hydrochloride and erythromycin, respectively. According to release exponent n, the release of lidocaine hydrochloride from the sheath part of the matrix is quasi-Fickian diffusion mechanism, while the release of erythromycin is based on anomalous or non-Fickian mechanisms.
通过静电纺丝法成功制备了基于聚（乙烯醇）（PVA）/壳聚糖/负载明胶纳米颗粒的盐酸利多卡因的纳米纤维核壳纳米复合双药递送系统。明胶纳米颗粒通过纳米沉淀法制备，然后负载平均粒径约为175 nm的红霉素抗菌剂。通过场发射扫描电子显微镜（FE-SEM）观察到，通过添加20 μL 5%的戊二醛作为交联剂，水相中明胶浓度为1.25 wt%时，明胶纳米颗粒的形态最佳。还通过动态光散射、zeta电位测量和傅里叶变换红外光谱（FTIR）对纳米颗粒进行了表征。PVA/壳聚糖纳米纤维垫在溶液重量比为96/4时获得了最佳的无珠形态。通过溶胀研究、FTIR和抗菌测试对纳米纤维垫进行了分析。还研究了核壳纳米纤维在72小时内的体外双释放曲线，结果表明盐酸利多卡因和红霉素的释放效率分别为84.69%和75.13%。根据释放指数n，盐酸利多卡因从基质鞘部的释放为准Fickian扩散机制，而红霉素的释放基于反常或非Fickian机制。
