CNC - Centro de Neurociências e Biologia Celular, CIBB - Centro de Inovação em Biomedicina e Biotecnologia, Universidade de Coimbra, Coimbra, Portugal.
Faculdade de Medicina, Universidade de Coimbra, Portugal.
Biomater Sci. 2021 Dec 7;9(24):8153-8159. doi: 10.1039/d1bm01034d.
Antimicrobial peptides (AMPs), such as LL37 peptides, may be immobilized on the surface of medical devices to render them with antimicrobial and angiogenic properties. However, little is known about LL37 properties after immobilization. Here, we have studied the antimicrobial and pro-angiogenic activity of soluble and immobilized LL37 peptides (conjugated to gold nanoparticles). Both soluble and immobilized LL37 peptides have potent antimicrobial activity against Gram-positive and Gram-negative bacteria in the presence of 10% human serum (HS). However, the immobilized LL37 peptides showed less cytotoxicity to endothelial cells (ECs) at a concentration that was able to kill bacteria. Interestingly, although both soluble and immobilized LL37 peptides showed pro-angiogenic activities in Matrigel and chorioallantoic membrane (CAM) assays, they induce different signalling pathways in ECs. The results described here highlight the importance of investigating the properties of immobilized AMPs that might act as a new entity.
抗菌肽 (AMPs)，如 LL37 肽，可固定在医疗器械表面，使其具有抗菌和促血管生成特性。然而，关于固定化后 LL37 的特性知之甚少。在这里，我们研究了可溶性和固定化 LL37 肽（与金纳米粒子偶联）的抗微生物和促血管生成活性。在存在 10%人血清 (HS) 的情况下，可溶性和固定化的 LL37 肽对革兰氏阳性和革兰氏阴性细菌均具有很强的抗菌活性。然而，在能够杀死细菌的浓度下，固定化的 LL37 肽对内皮细胞 (EC) 的细胞毒性较小。有趣的是，尽管可溶性和固定化的 LL37 肽在 Matrigel 和鸡胚尿囊膜 (CAM) 测定中均显示出促血管生成活性，但它们在 EC 中诱导不同的信号通路。这里描述的结果强调了研究可能作为新实体发挥作用的固定化 AMP 特性的重要性。
