Long non-coding RNA regulates the proliferation, migration, and apoptosis of esophageal cancer cells via the -miR-206- axis.

BACKGROUND

Esophageal cancer (EC) is a common malignant tumor of the digestive tract, the treatment of which involves surgery combined with radiotherapy and chemotherapy, as well as other comprehensive types of treatment. The pathogenesis of EC remains unclear, which hinders the development of clinical therapy and the identification of molecular targets for this disease. Long non-coding RNAs (lncRNAs) have been shown to be associated with the malignant biological behavior of EC, but the specific molecular mechanisms underlying the carcinogenesis of EC are not fully understood.

METHODS

Reverse transcription-quantitative PCR (RT-qPCR) was applied to measure the lncRNA HAGLR opposite strand lncRNA () levels in EC cell lines and tissues. Cell Counting Kit-8 (CCK-8) detection, scratch test, and Transwell assay were performed to determine the proliferation, migration and invasion of EC cell. The interaction between , microRNA (miR)-206, and notch receptor 3 () was confirmed by RNA immunoprecipitation and dual luciferase reporter gene assays.

RESULTS

is upregulated in esophageal squamous cell carcinoma (ESCC) tissues and predicts poor prognosis. Silent is negatively associated with malignant behavior in EC cells. Low expression of can induce decreased invasive and migratory abilities in EC cells. Downregulated significantly inhibits the proliferation of EC cells and accelerates apoptosis. promotes EC cell tumorigenesis . participates in the /miR-206/ regulatory axis in EC cells.

CONCLUSIONS

may play a role in the progression of EC by modulating the miR-206/ signaling axis, and may be a novel target for the diagnosis and treatment of EC.