Division of Phoniatrics, Medical University of Graz, Graz, Austria.
Diagnostic and Research Institute of Pathology, Diagnostic and Research Center of Molecular Medicine, Medical University of Graz, Graz, Austria.
Proteomics Clin Appl. 2022 Mar;16(2):e2100050. doi: 10.1002/prca.202100050. Epub 2021 Dec 1.
The vast majority of voice disorders is associated with changes of the unique, but delicate, human vocal fold mucosa. The ability to develop new effective treatment methods is significantly limited by the physical inaccessibility and the extremely rare occasions under which healthy tissue biopsies can be obtained. Therefore, the interest in laryngological research has shifted to human oral (buccal) mucosa, a similar and more easily available tissue. The harvesting process is less invasive and accompanied with faster healing and less scarring, compared to vocal fold mucosa. Here we report a descriptive proteomic comparison of paired human buccal and vocal fold mucosa by high-resolution mass spectrometry (CID-MS/MS). Our study identified a total of 1575 proteins detected within both tissues that are highly consistent in several crucial biological processes, cellular components, and molecular functions. Hence, our proteomic analysis will provide a fundamental resource for the laryngological research community.
绝大多数的嗓音障碍与独特但脆弱的人类声带黏膜的变化有关。由于组织的物理不可接近性以及健康组织活检的罕见性，开发新的有效治疗方法的能力受到了极大的限制。因此，喉科研究的兴趣已经转移到了人类口腔（颊）黏膜，这是一种相似且更容易获得的组织。与声带黏膜相比，该组织的采集过程创伤性更小，愈合更快，疤痕更少。在这里，我们通过高分辨率质谱（CID-MS/MS）报告了对配对的人颊黏膜和声带黏膜进行的描述性蛋白质组比较。我们的研究总共鉴定出了在两种组织中都检测到的 1575 种蛋白质，这些蛋白质在几个关键的生物学过程、细胞成分和分子功能中高度一致。因此，我们的蛋白质组学分析将为喉科研究界提供一个基本的资源。
