Universidad Autónoma de San Luis Potosí, Facultad de Estomatología, Grupo de Investigación en Ingeniería Tisular, San Luis Potosí, México.
Universidad Autónoma de San Luis Potosí, Facultad de Medicina, Maestría en Ciencias en Investigación Clínica, San Luis Potosí, México.
J Appl Oral Sci. 2017 Mar-Apr;25(2):186-195. doi: 10.1590/1678-77572016-0217.
The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot.
Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media. Substitutes were immunocharacterized with collagen IV and cytokeratin 5-14 as specific markers. A Student´s t- test was performed to assess the differences between both groups.
It was possible to isolate epidermal cells from the oral mucosa of diabetic and healthy subjects and develop autologous dermal-epidermal skin substitutes using autologous serum as a supplement. Differences in the expression of specific markers were observed and the cytokeratin 5-14 expression was lower in the diabetic substitutes, and the collagen IV expression was higher in the diabetic substitutes when compared with the healthy group, showing a significant difference.
Cells from oral mucosa could be an alternative and less invasive source for skin substitutes and wound healing. A difference in collagen production of diabetic cells suggests diabetic substitutes could improve diabetic wound healing. More research is needed to determine the crosstalk between components of these skin substitutes and damaged tissues.
本研究旨在从糖尿病患者的口腔黏膜中获得自体真皮-表皮皮肤替代物，作为糖尿病足患者临床应用的第一步。
从糖尿病患者和健康受试者（每组 20 例）中获得口腔黏膜。使用自体纤维蛋白分离和培养表皮细胞，通过空气-液体技术，用自体人血清作为补充培养基，开发自体真皮-表皮体外替代物。用胶原蛋白 IV 和细胞角蛋白 5-14 作为特异性标志物对替代物进行免疫鉴定。采用 Student's t 检验评估两组间的差异。
从糖尿病患者和健康受试者的口腔黏膜中分离出表皮细胞，并使用自体血清作为补充，成功开发出自体真皮-表皮皮肤替代物。观察到特异性标志物表达的差异，与健康组相比，糖尿病组的细胞角蛋白 5-14 表达较低，而胶原蛋白 IV 表达较高，差异具有统计学意义。
口腔黏膜细胞可能是皮肤替代物和伤口愈合的替代和微创来源。糖尿病细胞胶原产生的差异表明，糖尿病替代物可能改善糖尿病伤口愈合。需要进一步研究以确定这些皮肤替代物成分与受损组织之间的相互作用。
