Department of Biological Sciences, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa.
Department of Plant Production, University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.
Funct Plant Biol. 2021 Dec;49(1):13-24. doi: 10.1071/FP21148.
Interrogative proteome analyses are used to identify and quantify the expression of proteins involved in heat tolerance and to identify associated physiological processes in heat-stressed plants. The objectives of the study were to identify and quantify the expression of proteins involved in heat tolerance and to identify associated physiological processes in chickpea (Cicer arietinum L.) heat-tolerant (Acc#7) and sensitive genotype (Acc#8) from a field study. Proteomic and gene ontological analyses showed an upregulation in proteins related to protein synthesis, intracellular traffic, defence and transport in the heat-tolerant genotype compared to the susceptible one at the warmer site. Results from KEGG analyses indicate the involvement of probable sucrose-phosphate synthase (EC 2.4.1.14) and sucrose-phosphate phosphatase (EC 3.1.3.24) proteins, that were upregulated in the heat-tolerant genotype at the warmer site, in the starch and sucrose pathway. The presence of these differentially regulated proteins including HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin and protoporphyrinogen oxidase suggests their potential role in heat tolerance, at flowering growth stage, in field-grown chickpea. This observation supports unaltered physiological and biochemical performance of the heat-tolerant genotypes (Acc#7) relative to the susceptible genotype (Acc#8) in related studies (Makonya et al. 2019). Characterisation of the candidate proteins identified in the current study as well as their specific roles in the tolerance to heat stress in chickpea are integral to further crop improvement initiatives.
疑问蛋白组学分析用于鉴定和量化与耐热性相关的蛋白质的表达，并鉴定受热胁迫植物中的相关生理过程。本研究的目的是鉴定和量化与耐热性相关的蛋白质的表达，并鉴定在鹰嘴豆（Cicer arietinum L.）耐热（Acc#7）和敏感基因型（Acc#8）的田间研究中与耐热性相关的生理过程。蛋白质组学和基因本体分析表明，与蛋白质合成、细胞内运输、防御和运输相关的蛋白质在耐热基因型中上调，而在易感基因型中下调。KEGG 分析结果表明，可能涉及蔗糖磷酸合酶（EC 2.4.1.14）和蔗糖磷酸磷酸酶（EC 3.1.3.24）的蛋白，在温暖地区耐热基因型中上调，参与淀粉和蔗糖途径。这些差异调节蛋白的存在，包括 HSP70、核酮糖二磷酸羧化酶/加氧酶激活酶、质体蓝素和原卟啉原氧化酶，表明它们在开花生长阶段在田间生长的鹰嘴豆中耐热性的潜在作用。这一观察结果支持耐热基因型（Acc#7）相对于易感基因型（Acc#8）在相关研究中保持不变的生理和生化性能（Makonya 等人，2019 年）。当前研究中鉴定的候选蛋白的特征及其在鹰嘴豆耐热性中的特定作用是进一步作物改良计划的重要组成部分。
