Hsu S M, Ho Y S, Hsu P L
Department of Pathology, University of Texas Health Science Center, Houston 77225.
J Natl Cancer Inst. 1987 Nov;79(5):1091-9.
The interaction of 3 monoclonal antibodies (MAbs)--anti-HeFi-1, anti-2H9, and anti-IRac--with Reed-Sternberg (RS) cells was examined in vitro. MAb anti-2H9 induced specific modulation of the corresponding antigen (Ag) from the cell surface. Cell-bound anti-2H9 antibody (Ab)-Ag complexes rapidly disappeared during incubation of cells at 37 degrees C for 2-4 hours. This early loss was followed by reappearance of the Ag on the cell surface within 4-12 hours. In contrast, anti-HeFi-1 and anti-IRac bound avidly to the surface of RS cells and persisted for more than 3 days. During this period, there were no significant changes in the expression of HeFi-1 or IRac Ag on the surface of tumor cells. Cell surface-bound Abs were distributed uniformly, and there was no evidence of microaggregation, as determined by electron microscopy. None of the 3 MAbs was directly cytotoxic or exhibited complement-mediated cytotoxicity. On the basis of these findings (persistence of anti-HeFi-1 and anti-IRac on the cell surface), these 2 MAbs may be suitable for immunoimaging and immunotherapy for Hodgkin's disease.
在体外检测了3种单克隆抗体（MAb）——抗HeFi-1、抗2H9和抗IRac——与里德-施特恩伯格（RS）细胞的相互作用。单克隆抗体抗2H9可诱导相应抗原（Ag）从细胞表面特异性调节。细胞结合的抗2H9抗体（Ab）-Ag复合物在37℃孵育细胞2 - 4小时期间迅速消失。这种早期丢失之后，抗原在4 - 12小时内在细胞表面重新出现。相比之下，抗HeFi-1和抗IRac avidly结合到RS细胞表面并持续超过3天。在此期间，肿瘤细胞表面HeFi-1或IRac抗原的表达没有显著变化。通过电子显微镜确定，细胞表面结合的抗体均匀分布，没有微聚集的证据。这3种单克隆抗体均无直接细胞毒性或表现出补体介导的细胞毒性。基于这些发现（抗HeFi-1和抗IRac在细胞表面的持久性），这2种单克隆抗体可能适用于霍奇金病的免疫成像和免疫治疗。
