Hsu S M, Zhao X, Hsu P L, Lok M S
Am J Pathol. 1987 Apr;127(1):9-14.
Tumor cells from the Hodgkin's cell line HDLM-1 were cultured in extracellular matrix (ECM)-coated flasks. Within 24 hours, the cells underwent rapid differentiation, accompanied by morphologic and phenotypic changes. A slow and less prominent, but similar change was observed in HDLM-1 cells after they had been induced with phorbol ester for 5 days. The ECM-induced cells became HeFi-1-negative and expressed antigens 2H9 and 1E9 on their nuclear membranes. These cells had punctate acid-phosphatase and esterase activities. Approximately 70% of the ECM-induced cells were elongated and had long, blunt cytoplasmic projections. These findings, together with evidence the authors previously presented, indicate that H-RS cells are related to interdigitating reticulum cells. The authors believe that the ECM-induced HDLM-1 cells can be used as an important model for studies of the nature and cell lineage of Hodgkin's disease.
将霍奇金细胞系HDLM-1的肿瘤细胞培养在细胞外基质（ECM）包被的培养瓶中。在24小时内，细胞经历了快速分化，并伴有形态学和表型变化。在用佛波酯诱导5天后，HDLM-1细胞中观察到缓慢且不太明显但类似的变化。ECM诱导的细胞变为HeFi-1阴性，并在其核膜上表达抗原2H9和1E9。这些细胞具有点状酸性磷酸酶和酯酶活性。大约70%的ECM诱导细胞呈细长形，并有长而钝的细胞质突起。这些发现，连同作者之前提出的证据，表明霍奇金-里德-斯腾伯格（H-RS）细胞与交错突网状细胞有关。作者认为，ECM诱导的HDLM-1细胞可作为研究霍奇金病本质和细胞谱系的重要模型。
