from diffusion MRI reveals a correspondence between ventricular cerebrospinal fluid volume and flow in the ischemic rodent model.

Quantitative measurement of cerebrospinal fluid (CSF) flow and volume and longitudinal monitoring of CSF dynamics provide insights into the compensatory characteristics of post-stroke CSF. In this study, we compared the MRI pseudo-diffusion index () of live and sacrificed rat brains to confirm the effect of ventricular CSF flow on diffusion signals. We observed the relationship between the CSF peak velocities and through Monte Carlo (MC) simulations to further understand the source of contrast. We also determined the dominant CSF flow using in three directions. Finally, we investigated the dynamic evolutions of ventricular CSF flow and volume in a stroke rat model ( = 8) from preoperative to up to 45 days after surgery and determined the correlation between ventricular CSF volume and flow. MC simulations showed a strong positive correlation between the CSF peak velocity and ( = 0.99). The dominant CSF flow variations in the 3D ventricle could be measured using the maximum map. A longitudinal positive correlation between ventricular CSF volume and was observed in the lateral ( = 0.74) and ventral-third ( = 0.81) ventricles, respectively. The directional measurements provide quantitative CSF volume and flow information, which would provide useful insights into ischemic stroke with diffusion MRI.