Pei Haibing, Lin Zhiyin, Yao Kexin, Luo Yeqing, Tong Ping, Chen Hongbing, Wu Yong, Wu Zhihua, Gao Jinyan
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, 330047, P.R. China.
College of Food Science & Technology, Nanchang University, Nanchang, 330047, P.R. China.
J Physiol Biochem. 2024 Aug;80(3):541-559. doi: 10.1007/s13105-024-01021-2. Epub 2024 Apr 18.
Ovalbumin (OVA) is a major allergen in eggs and could induce severe allergic reactions in sensitive individuals, where the innate immune system works as a regulator. The mechanism of how innate immunity adjusts to food allergy is relatively well-studied, however, the effects of allergen uptake on the innate immune system remain unclear. Therefore, the Caenorhabditis elegans (C. elegans) model was utilized to assess the effects of OVA on its innate immune system. OVA enhanced the immune response of C. elegans with higher survival rates under Pseudomonas aeruginosa infection. Moreover, sustaining OVA treatment improved the health states that were reflected in the prolonged lifespan, alleviated oxidative stress, accelerated growth, and promoted motility. RNA-sequencing analysis and the slow-killing assays in the mutants of insulin/IGF-1 signaling (IIS)-related genes confirmed that IIS was necessary for OVA to regulate innate immunity. Besides, OVA activated SKN-1 temporarily and facilitated the nuclear localization of DAF-16 for improving immunity and health status in C. elegans. Together, OVA could enhance the innate immune responses via DAF-16 and SKN-1 pathways in the IIS of C. elegans, and this work will provide novel insights into the regulation of innate immunity by OVA in higher organisms.
卵清蛋白（OVA）是鸡蛋中的主要过敏原，可在敏感个体中引发严重的过敏反应，其中先天性免疫系统起着调节作用。虽然先天性免疫如何调节食物过敏的机制已得到较为充分的研究，但过敏原摄取对先天性免疫系统的影响仍不清楚。因此，利用秀丽隐杆线虫（C. elegans）模型评估OVA对其先天性免疫系统的影响。在铜绿假单胞菌感染下，OVA提高了秀丽隐杆线虫的免疫反应，使其存活率更高。此外，持续的OVA处理改善了健康状况，表现为寿命延长、氧化应激减轻、生长加速和运动能力增强。对胰岛素/胰岛素样生长因子-1信号（IIS）相关基因突变体的RNA测序分析和慢杀试验证实，IIS是OVA调节先天性免疫所必需的。此外，OVA可暂时激活SKN-1，并促进DAF-16的核定位，从而改善秀丽隐杆线虫的免疫力和健康状况。总之，OVA可通过秀丽隐杆线虫IIS中的DAF-16和SKN-1途径增强先天性免疫反应，这项工作将为高等生物中OVA对先天性免疫的调节提供新的见解。
