Wilson Mark R, Allard Marc W, Brown Eric W
Forensic Science Program, 325 Natural Science Bldg, Western Carolina University, Cullowhee, NC, 28723, USA.
Division of Microbiology (HFS-710), Center for Food Safety & Applied Nutrition, US Food & Drug Administration, 5100 Paint Branch Parkway, College Park, MD, USA.
Cladistics. 2013 Aug;29(4):449-461. doi: 10.1111/cla.12012. Epub 2013 Jan 21.
The forensic evaluation of bacterial pathogens presents new challenges to the forensic science community. This review examines bacterial pathogens as objects of forensic comparison, focusing on their nucleic acid sequences as an important aspect of the comparison process. Because of the clonal propagation of most bacterial pathogens, a phylogenetic approach to understanding the diversity and using this understanding to address common forensic questions is explored. As a general phylogenetic framework is now employed in human mitochondrial DNA analysis, we will use the relevant concepts and approaches common in this area to develop this approach further. We also address the impact of the current ease and prevalence of whole-genome DNA sequence analysis in the forensic comparison process.
对细菌病原体的法医鉴定给法医学界带来了新的挑战。本综述将细菌病原体作为法医比对的对象进行研究，重点关注其核酸序列，这是比对过程的一个重要方面。由于大多数细菌病原体的克隆繁殖，本文探讨了一种系统发育方法，以了解其多样性并利用这一认识来解决常见的法医问题。由于目前人类线粒体DNA分析中采用了一般的系统发育框架，我们将运用该领域常见的相关概念和方法来进一步发展这种方法。我们还讨论了全基因组DNA序列分析在法医比对过程中当前的便捷性和普遍性所带来的影响。
