Department of Functional Biology, University of Oviedo. C/Julian Claveria s/n, Oviedo 33006, Spain.
Int J Environ Res Public Health. 2013 Aug 29;10(9):3954-66. doi: 10.3390/ijerph10093954.
Brazilian freshwater fish caught from large drainages like the River Amazon represent a million ton market in expansion, which is of enormous importance for export to other continents as exotic seafood. A guarantee of bacteriological safety is required for international exports that comprise a set of different bacteria but not any Pseudomonas. However, diarrhoea, infections and even septicaemia caused by some Pseudomonas species have been reported, especially in immune-depressed patients. In this work we have employed PCR-based methodology for identifying Pseudomonas species in commercial fish caught from two different areas within the Amazon basin. Most fish caught from the downstream tributary River Tapajòs were contaminated by five different Pseudomonas species. All fish samples obtained from the River Negro tributary (Manaus markets) contained Pseudomonas, but a less diverse community with only two species. The most dangerous Pseudomonas species for human health, P. aeruginosa, was not found and consumption of these fish (from their Pseudomonas content) can be considered safe for healthy consumers. As a precautionary approach we suggest considering Pseudomonas in routine bacteriological surveys of imported seafood.
从亚马逊河等大型水系捕获的巴西淡水鱼代表着一个不断扩大的百万吨市场，作为外来海鲜出口到其他大陆具有巨大的重要性。国际出口需要保证细菌学安全，包括一组不同的细菌，但不包括任何假单胞菌。然而，一些假单胞菌物种引起的腹泻、感染甚至败血症已经有报道，特别是在免疫功能低下的患者中。在这项工作中，我们采用基于 PCR 的方法来鉴定从亚马逊盆地两个不同地区捕获的商业鱼类中的假单胞菌物种。从下游支流塔帕若斯河捕获的大多数鱼类都被五种不同的假单胞菌污染。从内格罗河支流（马瑙斯市场）获得的所有鱼类样本都含有假单胞菌，但种类较少，只有两种。对人类健康最危险的假单胞菌物种 P. aeruginosa 未被发现，因此对于健康消费者来说，食用这些鱼类（从其假单胞菌含量来看）是安全的。作为预防措施，我们建议在进口海鲜的常规细菌学调查中考虑假单胞菌。
