Physiology, University of Amazonia, BR-316, KM 03, Ananindeua, PA, 67113-901, Brazil.
Morphology and Genetics, Federal University of São Paulo, Rua Botucatu, 740, Vila Clementino, São Paulo, SP, 04023-900, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2021 Dec;872:503421. doi: 10.1016/j.mrgentox.2021.503421. Epub 2021 Nov 4.
We obtained peripheral blood lymphocyte samples from individuals occupationally exposed to X-rays in hospital radiology departments that use different radiology systems: analog film (AF), computerized radiology (CR), or digital radiology (DR). The micronucleus test (MNT) and comet assay were performed on the samples. Micronucleus cell counts (means vs. controls, i.e., individuals not occupationally exposed to ionizing radiation) were as follows: AF, 1.96 ± 0.21 vs 1.2 ± 0.25; CR, 1.89 ± 0.15 vs 1.31 ± 0.36; and DR, 1.75 ± 0.11 vs 1.59 ± 0.32. For the comet assay, damage scores were as follows; AF, 0.84 ± 0.22 vs 0.47 ± 0.04; CR, 0.64 ± 0.26 vs 0.43 ± 0.04; and DR, 0.56 ± 0.19 vs 0.49 ± 0035. These findings were consistent with cytogenetic damage due to radiation exposure.
我们从医院放射科使用不同放射系统（模拟胶片（AF）、计算机放射学（CR）或数字放射学（DR））的职业性 X 射线暴露个体中获得外周血淋巴细胞样本。对这些样本进行了微核试验（MNT）和彗星试验。微核细胞计数（与对照的平均值，即未接触电离辐射的个体）如下：AF，1.96±0.21 与 1.2±0.25；CR，1.89±0.15 与 1.31±0.36；DR，1.75±0.11 与 1.59±0.32。对于彗星试验，损伤评分如下：AF，0.84±0.22 与 0.47±0.04；CR，0.64±0.26 与 0.43±0.04；DR，0.56±0.19 与 0.49±0035。这些发现与辐射暴露引起的细胞遗传学损伤一致。
