Department of Regenerative Medicine, Cell and Tissue Bank, Chair of Urology and Andrology, Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9, 85-094, Bydgoszcz, Poland.
Department of Tissue Engineering, Chair of Urology and Andrology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.
Sci Rep. 2021 Nov 19;11(1):22614. doi: 10.1038/s41598-021-01996-8.
Mumio (Shilajit) is a traditional medicinal drug known and used for hundreds of years. Bladder cancer is one of the most common cancer types and better treatments are needed. This study analysed the in vitro effect of Mumio on urinary bladder cancer cells (T24 and 5637) in comparison to normal uroepithelial cells (SV-HUC1). Cytotoxicity of Mumio was analysed in these cell lines via MTT and real-time cell growth assays as well via the assessment of the cytoskeleton, apoptosis, and cell cycle. Mumio affected the viability of both cell types in a time and concentration dependent manner. We observed a selectivity of Mumio against cancer cells. Cell cycle and apoptosis analysis showed that Mumio inhibited G0/G1 or S phase cell cycle, which in turn induced apoptosis. Our results showed that Mumio was significantly more cytotoxic to urinary bladder cancer cells than to normal cells. These results are promising and indicate Mumio as a great candidate for urinary bladder cancer treatment and further investigations should be performed.
雪莲花（Mumio）是一种传统的药用药物，已有数百年的历史，被人们所了解和使用。膀胱癌是最常见的癌症类型之一，需要更好的治疗方法。本研究分析了 Mumio 对膀胱癌细胞（T24 和 5637）与正常尿路上皮细胞（SV-HUC1）的体外作用。通过 MTT 和实时细胞生长分析以及细胞骨架、细胞凋亡和细胞周期的评估，分析了 Mumio 在这些细胞系中的细胞毒性。Mumio 以时间和浓度依赖的方式影响两种细胞类型的活力。我们观察到 Mumio 对癌细胞具有选择性。细胞周期和细胞凋亡分析表明，Mumio 抑制 G0/G1 或 S 期细胞周期，进而诱导细胞凋亡。我们的结果表明，Mumio 对膀胱癌细胞的细胞毒性明显高于正常细胞。这些结果很有前途，表明 Mumio 是膀胱癌治疗的一个很好的候选药物，应该进行进一步的研究。
