头颈部鳞状细胞癌中动态对比增强 MRI 与肿瘤浸润淋巴细胞和肿瘤间质比的相关性。

Associations between dynamic-contrast enhanced MRI and tumor infiltrating lymphocytes and tumor-stroma ratio in head and neck squamous cell cancer.

机构信息

Department of Diagnostic and Interventional Radiology, University of Leipzig, Leipzig, Germany.

Department of Pathology, University of Leipzig, Leipzig, Germany.

出版信息

Cancer Imaging. 2021 Nov 20;21(1):60. doi: 10.1186/s40644-021-00429-z.

OBJECTIVES

The present study used dynamic-contrast enhanced MRI (DCE-MRI) to elucidate possible associations with tumor-infiltrating lymphocytes (TIL), stroma ratio and vimentin expression in head and neck squamous cell cancer (HNSCC).

METHODS

Overall, 26 patients with primary HNSCC of different localizations were involved in the study. DCE-MRI was obtained on a 3 T MRI and analyzed with a whole lesion measurement using a histogram approach. TIL- and vimentin-expression was calculated on bioptic samples before any form of treatment. P16 staining was used to define HPV-status.

RESULTS

Tumor-stroma ratio correlated with entropy derived from K (r = - 0.52, p = 0.0071) and with kurtosis derived from V (r = - 0.53, p = 0.0058). Several V derived parameters correlated with expression of TIL within the stroma compartment. TIL within the tumor compartment correlated with entropy derived from K (r = 0.39, p = 0.047), p90 derived from V (r = 0.41, p = 0.036) and skewness derived from V (r = 0.41, p = 0.037). Furthermore, these associations were different between HPV positive and negative tumors.

CONCLUSIONS

DCE-MRI might be able to reflect tumor compartments and TIL expression in HNSCC. The most promising parameters were values derived from K and V.

目的

本研究采用动态对比增强 MRI（DCE-MRI）来阐明头颈部鳞状细胞癌（HNSCC）中肿瘤浸润淋巴细胞（TIL）、基质比和波形蛋白表达与肿瘤之间的可能相关性。

方法

共有 26 名不同部位原发性 HNSCC 患者参与了这项研究。在 3T MRI 上获得 DCE-MRI，并使用全病变测量方法通过直方图分析进行分析。在任何治疗形式之前，通过活检样本计算 TIL 和波形蛋白表达。P16 染色用于定义 HPV 状态。

结果

肿瘤-基质比与 K 衍生的熵（r = -0.52，p = 0.0071）和 V 衍生的峰度（r = -0.53，p = 0.0058）相关。几个 V 衍生参数与基质内 TIL 的表达相关。肿瘤内 TIL 与 K 衍生的熵（r = 0.39，p = 0.047）、V 衍生的 p90（r = 0.41，p = 0.036）和 V 衍生的偏度（r = 0.41，p = 0.037）相关。此外，这些相关性在 HPV 阳性和阴性肿瘤之间存在差异。