Ch'ng Angela Chiew Wen, Lam Paula, Alassiri Mohammed, Lim Theam Soon
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia.
CellVec Private Limited, 118518, Singapore; National University of Singapore, Department of Physiology, 117597, Singapore; Duke-NUS Graduate Medical School, Cancer and Stem Cells Biology Program, 169857, Singapore.
Biotechnol Adv. 2022 Jan-Feb;54:107870. doi: 10.1016/j.biotechadv.2021.107870. Epub 2021 Nov 18.
The immune system is tasked to keep our body unharmed and healthy. In the immune system, B- and T-lymphocytes are the two main components working together to stop and eliminate invading threats like virus particles, bacteria, fungi and parasite from attacking our healthy cells. The function of antibodies is relatively more direct in target recognition as compared to T-cell receptors (TCR) which recognizes antigenic peptides being presented on the major histocompatibility complex (MHC). Although phage display has been widely applied for antibody presentation, this is the opposite in the case of TCR. The cell surface TCR is a relatively large and complex molecule, making presentation on phage surfaces challenging. Even so, recombinant versions and modifications have been introduced to allow the growing development of TCR in phage display. In addition, the increasing application of TCR for immunotherapy has made it an important binding motif to be developed by phage display. This review will emphasize on the application of phage display for TCR discovery as well as the engineering aspect of TCR for improved characteristics.
免疫系统的任务是保护我们的身体不受伤害并保持健康。在免疫系统中，B淋巴细胞和T淋巴细胞是两个主要组成部分，它们协同工作以阻止并消除诸如病毒颗粒、细菌、真菌和寄生虫等入侵威胁对我们健康细胞的攻击。与识别呈递于主要组织相容性复合体（MHC）上的抗原肽的T细胞受体（TCR）相比，抗体在目标识别方面的功能相对更直接。尽管噬菌体展示已广泛应用于抗体呈递，但在TCR的情况中却恰恰相反。细胞表面的TCR是一个相对较大且复杂的分子，这使得在噬菌体表面进行呈递具有挑战性。即便如此，人们已经引入了重组版本和修饰方法，以促进TCR在噬菌体展示中的不断发展。此外，TCR在免疫疗法中的应用日益增加，这使其成为噬菌体展示要开发的一种重要结合基序。本综述将重点关注噬菌体展示在TCR发现中的应用以及TCR为改善特性而进行的工程改造方面。
