School of Ecology and Environment, Inner Mongolia Key Laboratory of Environmental Pollution Control & Wastes Reuse, Inner Mongolia University, Hohhot 010021, China; Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996, United States.
Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996, United States.
Bioresour Technol. 2022 Mar;347:126367. doi: 10.1016/j.biortech.2021.126367. Epub 2021 Nov 18.
Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 × 10 CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.
共溶剂增强的木质纤维素分级（CELF）是一种新兴的溶剂解预处理方法，可用于分离木质纤维素生物质。本文首次全面评估了 CELF 木质素的生物转化性能。结果表明，与其他两种技术木质素（包括常规乙醇有机溶剂木质素（EOL）和 kraft 木质素（KL））相比，CELF 木质素具有更高的羧酸 OH 含量、更低的分子量和消失的 β-O-4 和 β-5 键。从 CELF 木质素发酵中获得的 Rhodococcus opacus PD630 细胞计数达到了最高值 3.9×10 CFU/mL，分别比 EOL 和 KL 提高了 62.5%和 77.3%。相应地，从 CELF 木质素中获得的脂质产量达到 143mg/L，分别比 EOL 和 KL 提高了 36.2%和 26.5%。主成分分析（PCA）表明，更多的羧酸基团和更低的分子量有助于提高 CELF 木质素的生物转化性能。本研究表明，CELF 木质素是一种很有前途的生物转化候选物。
