Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, Sweden.
Chalmers University of Technology, Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Kemivägen 10, SE-412 96 Göteborg, Sweden.
Bioresour Technol. 2019 Feb;273:521-528. doi: 10.1016/j.biortech.2018.11.055. Epub 2018 Nov 15.
For a transition to a sustainable society, fuels, chemicals, and materials should be produced from renewable resources. Lignocellulosic biomass constitutes an abundant and renewable feedstock; however, its successful application in a biorefinery requires efficient fractionation into its components; cellulose, hemicellulose and lignin. Here, we demonstrate that a newly established hybrid organosolv - steam explosion pretreatment can effectively fractionate spruce biomass to yield pretreated solids with high cellulose (72% w/w) and low lignin (delignification up to 79.4% w/w) content. The cellulose-rich pretreated solids present high saccharification yields (up to 61% w/w) making them ideal for subsequent bioconversion processes. Moreover, under high-gravity conditions (22% w/w) we obtained an ethanol titer of 61.7 g/L, the highest so far reported for spruce biomass. Finally, the obtained high-purity lignin is suitable for various advanced applications. In conclusion, hybrid organosolv pretreatment could offer a closed-loop biorefinery while simultaneously adding value to all biomass components.
为了向可持续社会过渡，燃料、化学品和材料应该由可再生资源生产。木质纤维素生物质构成了丰富且可再生的原料；然而，要将其成功应用于生物炼制厂，需要将其有效分离成纤维素、半纤维素和木质素等成分。在这里，我们证明了一种新建立的混合有机溶剂-蒸汽爆破预处理方法可以有效地将云杉生物质分离成预处理后的固体，具有高纤维素（72%w/w）和低木质素（脱木质素高达 79.4%w/w）含量。富含纤维素的预处理固体具有很高的糖化产率（高达 61%w/w），非常适合后续的生物转化过程。此外，在高重力条件下（22%w/w），我们获得了 61.7 g/L 的乙醇浓度，这是迄今为止报道的云杉生物质的最高浓度。最后，获得的高纯度木质素适用于各种先进的应用。总之，混合有机溶剂预处理可以提供一个闭环生物炼制厂，同时为所有生物质成分增加价值。
