School of Food Science and Technology, Dalian Polytechnic University, Dalian, China.
National Engineering Research Center of Seafood, Dalian, China.
J Sci Food Agric. 2022 Jun;102(8):3277-3286. doi: 10.1002/jsfa.11673. Epub 2021 Dec 6.
Zinc (Zn) is an essential catalytic element in the human health system but its absorption in the intestinal system can be strongly affected by gastrointestinal (GI) digestion. In this study, the food-derived potential Zn carrier, scallop adductor hydrolysates (SAHs), was produced and characterized.
During temporary storage at 4 °C, SAH decreased in Zn-chelating capacity in the aqueous phase, whereas the SAH-Zn complex exhibited high stability. Moreover, the secondary structure of SAH had no significant alteration. Zn morphologically altered the surface structures of SAH, which was involving in carboxyl group of SAH. Results of in vitro GI digestion suggested that the SAH-Zn maintained good stability in GI system and only proportion of high molecular weight cleaved. In addition, SAH could successfully carry and transport Zn while the fluorescence staining revealed free Zn accumulation inside the tissue. Finally, three representative absorbed peptides (around 600 Da) were identified and synthesized. Three synthetic peptides exhibit higher Zn-chelating capacity than SAH and could also successfully transported through the intestine.
This study provided a theoretical basis for the investigation of digestion and absorption of marine animal-derived peptides as Zn carriers. © 2021 Society of Chemical Industry.
锌（Zn）是人体健康系统中必不可少的催化元素，但它在肠道系统中的吸收会受到胃肠道（GI）消化的强烈影响。在这项研究中，开发并鉴定了贝类肌肉水解物（SAH）这一具有食物来源的潜在 Zn 载体。
在 4°C 临时储存期间，SAH 在水相中的 Zn 结合能力下降，而 SAH-Zn 复合物表现出高稳定性。此外，SAH 的二级结构没有明显改变。Zn 改变了 SAH 的表面结构，这涉及 SAH 的羧基。体外 GI 消化结果表明，SAH-Zn 在 GI 系统中保持良好的稳定性，只有部分高分子量片段被水解。此外，SAH 能够成功地携带和转运 Zn，荧光染色显示组织内有游离 Zn 积累。最后，鉴定并合成了三个具有代表性的吸收肽（约 600Da）。这三个合成肽具有比 SAH 更高的 Zn 结合能力，也可以成功地穿过肠壁。
本研究为研究海洋动物衍生肽作为 Zn 载体的消化和吸收提供了理论依据。 © 2021 英国化学学会。
