Department of Medicinal Chemistry, School of Pharmacy, Qingdao University, No.38 Dengzhou Road, Qingdao, Shandong Province 266021, PR China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, No.106 Nanjing Road, Qingdao, Shandong Province 266071, PR China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, No.106 Nanjing Road, Qingdao, Shandong Province 266071, PR China.
Food Chem. 2021 Mar 15;340:128056. doi: 10.1016/j.foodchem.2020.128056. Epub 2020 Sep 25.
In the study, a novel kind of peptides-zinc (AKP-Zn) chelate was obtained using the Antarctic krill (Euphausia superba) peptides (AKP) as raw material, the reaction was carried out with the mass ratio of the AKP to ZnSO·7HO of 1:2 at pH 6.0 and 60 °C for 10 min. The structure and composition of the AKP, including particle size, Zeta potential, molecular weight distribution, amino acid composition, microstructure and surface elemental composition, changed significantly after chelating with zinc. The result of Fourier transform infrared spectroscopy indicated that zinc could be chelated by carboxyl oxygen and amino nitrogen atoms of the AKP. Furthermore, compared with zinc sulfate and zinc gluconate, the AKP-Zn chelate was more stable at various pH conditions and the simulated gastrointestinal digestion experiment. These findings would provide a scientific basis for developing new zinc supplements and the high-value utilization of Antarctic krill protein resource.
在研究中，利用南极磷虾肽（AKP）作为原料，获得了一种新型的肽-锌（AKP-Zn）螯合物。该反应在 AKP 与 ZnSO·7HO 的质量比为 1:2、pH 值为 6.0 和 60°C 的条件下进行了 10 分钟。AKP 的结构和组成，包括粒径、Zeta 电位、分子量分布、氨基酸组成、微观结构和表面元素组成，在与锌螯合后发生了显著变化。傅里叶变换红外光谱的结果表明，锌可以与 AKP 的羧基氧和氨基氮原子螯合。此外，与硫酸锌和葡萄糖酸锌相比，AKP-Zn 螯合物在各种 pH 条件和模拟胃肠道消化实验中更稳定。这些发现为开发新型锌补充剂和南极磷虾蛋白资源的高值利用提供了科学依据。
