Lisowska Katarzyna A, Storoniak Hanna, Dębska-Ślizień Alicja
Department of Pathophysiology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.
Department of Nephrology, Transplantology and Internal Medicine, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.
Hum Immunol. 2022 Feb;83(2):134-143. doi: 10.1016/j.humimm.2021.11.003. Epub 2021 Nov 18.
HD patients have impaired adaptive immune responses, which might depend on the primary cause of chronic kidney disease (CKD). We analyzed percentages of T cells subpopulations with the expression of CD69, CD25, CD95, and HLA-DR antigens in HD patients to determine the status of T cell activation. Also, we determined serum levels of cytokines: IL12p70, TNF, IL-10, IL-6, IL-1β, IL-8. HD patients had increased percentages of CD4CD25, CD4CD69, CD4HLA-DR, CD8CD69, and CD8HLA-DR cells compared to healthy people. Also, their IL-6 and IL-8 serum levels were higher. Changes in T cell subpopulations were seen in patients with diabetic nephropathy (DN) or ischemic nephropathy (IN) but not with glomerulonephritis (GN). HD patients dialyzed for more than six months had a lower percentage of CD4CD69, CD8HLA-DR, CD8CD95 cells, higher IL-12p70 levels, and lower IL-8 levels. Our results show that HD treatment and CKD cause influence T cell activation status.
血液透析患者的适应性免疫反应受损，这可能取决于慢性肾脏病（CKD）的主要病因。我们分析了血液透析患者中表达CD69、CD25、CD95和HLA - DR抗原的T细胞亚群百分比，以确定T细胞的激活状态。此外，我们还测定了细胞因子的血清水平：IL12p70、TNF、IL - 10、IL - 6、IL - 1β、IL - 8。与健康人相比，血液透析患者的CD4CD25、CD4CD69、CD4HLA - DR、CD8CD69和CD8HLA - DR细胞百分比增加。此外，他们的IL - 6和IL - 8血清水平更高。在糖尿病肾病（DN）或缺血性肾病（IN）患者中观察到T细胞亚群的变化，但在肾小球肾炎（GN）患者中未观察到。透析超过六个月的血液透析患者的CD4CD69、CD8HLA - DR、CD8CD95细胞百分比更低，IL - 12p70水平更高，IL - 8水平更低。我们的结果表明，血液透析治疗和慢性肾脏病会影响T细胞的激活状态。
