Almeida A, Lourenço O, Fonseca A M
CICS-UBI, Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal.
Scand J Immunol. 2015 Aug;82(2):135-41. doi: 10.1111/sji.12309.
Diabetic nephropathy (DN) is a common complication in patients with diabetes, and most of them need renal replacement therapy such as haemodialysis (HD). These patients have a high tendency to develop infections and exhibit anomalies in the immune system. The objective of this study was to assess the expression of activation-related markers on T cells, as well as to quantify inflammatory cytokines, before and after a single HD session in DN patients. The study involved DN patients under HD treatment who signed an informed consent form. Blood samples before and after one HD session were collected, to analyse the expression of CD25, CD69 and CD71 in T cells. We also quantified IL-12p70, IL-8, IL-10, IL-1β, TNF-α and IL-6 in serum samples using the cytometric bead array technique. After the HD session, there was an increase in the CD4/CD8 ratio due to significant alterations in both subsets. The relative percentage of CD25+ cells and CD8+ CD25+ increased significantly after the HD session, while the relative percentage of CD69 T cells decreased. There was a significant decrease in the CD25 mean fluorescence intensity values for CD4+ T, as well as in the case of CD71 in T cells after the HD session. Regarding cytokine synthesis, we found a significant increase in IL-10 and IL-6 and a decrease in IL-8 after HD session. This study showed that a HD session in DN patients affects the T cell activation status in the two major subpopulations and differentially modulates the production of inflammatory cytokines.
糖尿病肾病（DN）是糖尿病患者常见的并发症，其中大多数患者需要进行血液透析（HD）等肾脏替代治疗。这些患者发生感染的倾向较高，且免疫系统存在异常。本研究的目的是评估DN患者单次HD治疗前后T细胞上激活相关标志物的表达，以及对炎性细胞因子进行定量分析。该研究纳入了签署知情同意书的接受HD治疗的DN患者。采集一次HD治疗前后的血样，以分析T细胞中CD25、CD69和CD71的表达。我们还使用细胞计数珠阵列技术对血清样本中的IL-12p70、IL-8、IL-10、IL-1β、TNF-α和IL-6进行了定量分析。HD治疗后，由于两个亚群均发生显著变化，CD4/CD8比值升高。HD治疗后，CD25+细胞和CD8+CD25+的相对百分比显著增加，而CD69 T细胞的相对百分比降低。HD治疗后，CD4+T细胞的CD25平均荧光强度值以及T细胞中CD71的平均荧光强度值均显著降低。关于细胞因子的合成，我们发现HD治疗后IL-10和IL-6显著增加，IL-8减少。本研究表明，DN患者的一次HD治疗会影响两个主要亚群的T细胞激活状态，并对炎性细胞因子的产生进行不同程度的调节。
