Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.
Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120617. doi: 10.1016/j.saa.2021.120617. Epub 2021 Nov 13.
Herein, a fluorescent nanosensor has been constructed for detection of glutathione (GSH) based on NH-UiO-66 and AgNPs. The NH-UiO-66 was synthesized where 2-amino-terephthalic acid as the organic ligand and Zr as the center metal ions. The AgNPs can enhance the fluorescence of NH-UiO-66 based on metal enhanced fluorescence (MEF) effect. Moreover, in the present of GSH, the fluorescence of NH-UiO-66@AgNPs was quenched via electrostatic interaction and Ag-S reaction. The present sensing strategy shows good linear relation with the concentration of GSH in the range of 0.2-1.0 μM and 1.0-30 μM, and the limit of detection is 79 nM. Furthermore, our fluorescent nanosensor was utilized to detect GSH in human serum with a recovery of 96.8-102.5%. The results indicated that NH-UiO-66@AgNPs is successfully applied for high sensitive and selective detection of GSH in human serum.
本文构建了一种基于 NH-UiO-66 和 AgNPs 的荧光纳米传感器，用于检测谷胱甘肽（GSH）。NH-UiO-66 是通过使用 2-氨基对苯二甲酸作为有机配体和 Zr 作为中心金属离子合成的。AgNPs 可以基于金属增强荧光（MEF）效应增强 NH-UiO-66 的荧光。此外，在 GSH 的存在下，NH-UiO-66@AgNPs 的荧光通过静电相互作用和 Ag-S 反应被猝灭。该传感策略在 0.2-1.0 μM 和 1.0-30 μM 的范围内表现出与 GSH 浓度良好的线性关系，检测限为 79 nM。此外，我们的荧光纳米传感器用于检测人血清中的 GSH，回收率为 96.8-102.5%。结果表明，NH-UiO-66@AgNPs 成功应用于人血清中 GSH 的高灵敏和选择性检测。
