State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Analyst. 2022 Aug 22;147(17):3867-3875. doi: 10.1039/d2an00944g.
Hypochlorite plays a vital role in biological systems and our daily life. The rapid and convenient detection of hypochlorite is imperative and significant for disease treatment and human health. In this work, EY/UiO-66-NH (EY = eosin Y) was prepared through a hydrothermal process and could be applied to the detection and bioimaging of hypochlorite as a self-calibrating sensing nanoprobe. EY/UiO-66-NH features two emissions at 432 nm and 533 nm, and the emission intensity of 533 nm is enhanced with increasing ClO concentration. EY/UiO-66-NH could be utilized as a ratiometric fluorescence sensor of ClO. The linear range of EY/UiO-66-NH towards ClO is 0.1-200 μM and its detection limit is 46.4 nM. In comparison with previously reported probes for ClO, EY/UiO-66-NH has the advantages of a wide linear range, low detection limit, turn-on fluorescence and ratiometric response. This work provides a new method for ClO detection in living cells.
次氯酸盐在生物系统和日常生活中起着至关重要的作用。快速便捷地检测次氯酸盐对于疾病治疗和人类健康具有重要意义。在这项工作中，通过水热法制备了 EY/UiO-66-NH（EY = 曙红 Y），可作为一种自校准传感纳米探针，用于次氯酸盐的检测和生物成像。EY/UiO-66-NH 在 432nm 和 533nm 处具有两个发射峰，并且随着 ClO 浓度的增加，533nm 的发射强度增强。EY/UiO-66-NH 可用作 ClO 的比率荧光传感器。EY/UiO-66-NH 对 ClO 的线性范围为 0.1-200μM，检测限为 46.4nM。与之前报道的用于 ClO 的探针相比，EY/UiO-66-NH 具有线性范围宽、检测限低、荧光开启和比率响应的优点。这项工作为活细胞中 ClO 的检测提供了一种新方法。
