Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, China.
Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction and Guangdong-Hong Kong-Macao Joint Laboratory of Optoelectronic and Magnetic Functional Materials, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
Biosens Bioelectron. 2022 Nov 1;215:114582. doi: 10.1016/j.bios.2022.114582. Epub 2022 Jul 21.
The discriminative detection of glutathione (GSH) from cysteine (Cys) remains a challenge because of their similarity in structure and chemical properties. This study reported a strategy for selective and sensitive detection of GSH based on the GSH-promoted blue fluorescence of UiO-66-NH and aggregation-enhanced emission (AEE) feature of orange emissive Cu nanoclusters (NCs). A relatively weak blue fluorescence of UiO-66-NH was converted to strong after reacting with GSH due to the rotation-restricted emission enhancement mechanism. In addition, the GSH-activated UiO-66-NH was further used as a template and reducing reagent for synthesizing orange-red AEE active Cu NCs composites (UiO-66-NH@Cu NCs). A ratiometric fluorescence response was observed after forming UiO-66-NH@Cu NCs, helping discriminate GSH over Cys. In addition, UiO-66-NH@Cu NCs were further utilized for the detection of GSH in clinical samples. The present findings provide an efficient strategy to discriminate GSH over Cys and open a new door for utilizing and functionalizing metal-organic frameworks (MOFs) for various applications.
由于谷胱甘肽（GSH）和半胱氨酸（Cys）在结构和化学性质上的相似性，对它们进行区分检测仍然是一个挑战。本研究报道了一种基于 UiO-66-NH 中谷胱甘肽促进的蓝色荧光和橙色发射铜纳米团簇（NCs）的聚集增强发射（AEE）特性，用于选择性和灵敏检测 GSH 的策略。由于旋转受限发射增强机制，UiO-66-NH 与 GSH 反应后，相对较弱的蓝色荧光转变为强荧光。此外，GSH 激活的 UiO-66-NH 进一步用作模板和还原剂，用于合成橙色红色 AEE 活性 Cu NCs 复合材料（UiO-66-NH@Cu NCs）。形成 UiO-66-NH@Cu NCs 后观察到比率荧光响应，有助于区分 GSH 和 Cys。此外，UiO-66-NH@Cu NCs 进一步用于临床样本中 GSH 的检测。本研究结果为区分 GSH 和 Cys 提供了一种有效的策略，并为金属有机骨架（MOFs）的各种应用的利用和功能化开辟了新的途径。
