Department of Oncology Surgery, The Second Affiliated Hospital of Qiqihaer Medical College, Qiqihar, Heilongjiang Province, China.
College of Pharmacy, Qiqihar Medical University, Qiqihar, Heilongjiang Province, China.
Comput Math Methods Med. 2021 Nov 11;2021:9571286. doi: 10.1155/2021/9571286. eCollection 2021.
With the increasing resistance of antibiotics to bacteria, new and effective methods are needed to transform existing antibiotics to solve the problem of long development cycles for new drugs. The antibiotic nanodelivery system has proven to be a promising strategy.
The purpose of this study is to synthesize penicillin solid lipid nanoparticles (penicillin SLNs) to enhance the antibacterial activity of penicillin against drug-resistant Staphylococcus aureus.
Penicillin SLNs were synthesized. And particle size, the polydispersity index (PI), and zeta potential (ZP) of penicillin SLNs were measured. The surface morphology of penicillin SLNs was observed using a transmission electron microscope.
The particle size of penicillin SLNs is 112.3 ± 11.9 nm, the polydispersity index (PI) and zeta potential (ZP) of penicillin SLNs are 0.212 ± 0.03 and -27.6 ± 5.5 mV. The encapsulation efficiency and drug loading were 98.31 ± 1.2% and 4.98 ± 0.05 (%/), respectively. Penicillin SLNs had a more significant inhibitory effect on the growth of methicillin-sensitive Staphylococcus aureus (MSSA) after the drug and the bacteria were incubated for 12 hours. The number of MRSA colonies in the penicillin group increased after 12 hours, while the number of MRSA colonies in the penicillin SLNs group did not change significantly.
Penicillin SLNs enhance the ability of penicillin to enter cells and increase the concentration of penicillin in the cell and also extend the residence time of penicillin in the cell. Our findings indicated that penicillin SLNs enhance the inhibitory effect of penicillin on drug-resistant Staphylococcus aureus.
随着抗生素对细菌的耐药性不断增加，需要新的有效方法来改造现有的抗生素，以解决新药开发周期长的问题。抗生素纳米递药系统已被证明是一种很有前途的策略。
本研究旨在合成青霉素固体脂质纳米粒（penicillin SLNs），以增强青霉素对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性。
合成青霉素 SLNs，并测量其粒径、多分散指数（PI）和 Zeta 电位（ZP）。使用透射电子显微镜观察青霉素 SLNs 的表面形态。
青霉素 SLNs 的粒径为 112.3±11.9nm，多分散指数（PI）和 Zeta 电位（ZP）分别为 0.212±0.03 和-27.6±5.5mV。包封率和载药量分别为 98.31±1.2%和 4.98±0.05（%/）。药物与细菌孵育 12 小时后，青霉素 SLNs 对甲氧西林敏感金黄色葡萄球菌（MSSA）的生长抑制作用更为明显。青霉素组的 MRSA 菌落数在 12 小时后增加，而青霉素 SLNs 组的 MRSA 菌落数没有明显变化。
青霉素 SLNs 增强了青霉素进入细胞的能力，增加了细胞内青霉素的浓度，并延长了青霉素在细胞内的停留时间。我们的研究结果表明，青霉素 SLNs 增强了青霉素对耐甲氧西林金黄色葡萄球菌的抑制作用。
