Faculty of Life and Environmental Science, University of Yamanashi, Kofu 400-8510, Japan.
Advanced Biotechnology Center, University of Yamanashi, Kofu 400-8510, Japan.
STAR Protoc. 2021 Nov 3;2(4):100933. doi: 10.1016/j.xpro.2021.100933. eCollection 2021 Dec 17.
The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse strains in a card folder. We can also send the FD sperm domestically using a postcard without any extra equipment. For complete details on the use and execution of this protocol, please refer to Ito et al. (2021).
哺乳动物冻干（FD）精子的保存通常使用小玻璃安瓿进行；然而，它们体积庞大且易碎。在这项研究中，我们提出了一种使用薄塑料片制备和保存小鼠 FD 精子的方案。这种方法允许在卡片文件夹中储存数千种小鼠品系。我们还可以使用明信片在国内发送 FD 精子，而无需任何额外的设备。有关该方案使用和执行的完整详细信息，请参阅 Ito 等人（2021 年）。
