Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Medinah, Saudi Arabia.
Luminescence. 2022 Feb;37(2):255-262. doi: 10.1002/bio.4168. Epub 2021 Dec 1.
A new sensitive and instantaneous spectrofluorimetric method for efficient determination of lomefloxacin (LMX) in its pure, dosage form and human plasma was designed. The developed method depends on formation of a metal-chelation compound of LMX as a ligand with zinc(II) in a buffer of acetate (pH 5.5). The following parameters; type of metal, concentration of metal, pH, type of buffer and diluting solvent were optimized. After carefully investigation; 0.2 mM zinc, 2.0 ml acetate buffer (pH 5.5) and water as diluting solvent were set as optimum reaction conditions. Under these conditions, a large increase in the intensity of the fluorescence of LMX was attained at 450 after excitation at 284 nm. The limits of detection and quantification were 5.8 and 1.9 ng ml , respectively, with linearity range of 10.0 to 500.0 ng ml . The binding mode of LMX and zinc(II) ion (Zn ) was found to be 2:1, respectively, and confirmed by Job's plot method. Furthermore, it extended to the analysis of LMX in the spiked plasma of humans with percentage recovery (98.70 ± 0.97 to 100.30 ± 1.69%, n = 3).
设计了一种新的灵敏、瞬时分光荧光法，用于高效测定洛美沙星（LMX）的纯品、制剂和人血浆中的含量。该方法依赖于 LMX 作为配体与醋酸盐缓冲液（pH 5.5）中的锌（II）形成金属螯合物。优化了以下参数：金属类型、金属浓度、pH 值、缓冲液类型和稀释溶剂。经过仔细研究；选择 0.2 mM 的锌、2.0 ml 的醋酸盐缓冲液（pH 5.5）和水作为稀释溶剂作为最佳反应条件。在这些条件下，在 284nm 激发后，LMX 的荧光强度在 450 处大幅增加。检测限和定量限分别为 5.8 和 1.9 ng·ml-1，线性范围为 10.0 至 500.0 ng·ml-1。通过 Job 图谱法发现 LMX 和锌（II）离子（Zn2+）的结合模式分别为 2:1，并得到了证实。此外，它还扩展到了人血浆中添加 LMX 的分析，回收率为 98.70±0.97%至 100.30±1.69%（n=3）。
