Derayea Sayed M, Hassan Yasser F, Hammad Mohamed A, Alahmadi Yasser M, Omar Mahmoud A, Samir Ebtihal
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut branch, Assiut 71524, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 May 5;292:122399. doi: 10.1016/j.saa.2023.122399. Epub 2023 Jan 30.
The purpose of the present work was to establish a fast and convenient strategy for lomefloxacin analysis using a fluorimetric approach. The methodology was based on the complex formation of the drug with aluminum ion to give a product having high fluorescence. Adding sodium dodecyl sulfate led to further boosting the intensity of fluorescence which was recorded at 429 nm after excitation at 332 nm. The relationship of emission intensity with lomefloxacin concentration was linear at 10-130 ng mL with a correlation coefficient of 0.9996. The quantitation limit was 11.4 ng mL and detection limit was 3.8 ng mL. The reaction conditions were carefully studied which included the pH, buffer type, its concentration, the type and concentration of surfactant and the diluting solvent. The method was utilized to quantify the aforementioned drug in tablet formulations and in real human plasma with high accuracy and reliability.
本研究的目的是建立一种快速便捷的洛美沙星荧光分析法。该方法基于药物与铝离子形成络合物，产生具有高荧光的产物。加入十二烷基硫酸钠可进一步增强荧光强度，在332nm激发后于429nm处记录荧光强度。在10 - 130ng/mL范围内，发射强度与洛美沙星浓度呈线性关系，相关系数为0.9996。定量限为11.4ng/mL，检测限为3.8ng/mL。对反应条件进行了仔细研究，包括pH值、缓冲液类型、其浓度、表面活性剂的类型和浓度以及稀释溶剂。该方法用于准确可靠地定量片剂制剂和实际人血浆中的上述药物。
