Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.
Abbott, Santa Clara, California, USA.
Int J Lab Hematol. 2022 Apr;44(2):288-295. doi: 10.1111/ijlh.13764. Epub 2021 Nov 22.
White blood cell (WBC) differential by flow cytometry can report a six-part WBC differential and enumerate blasts. Some modern hematology analyzers are also able to provide a six-part WBC differential (including immature granulocytes). Our goal was to compare the WBC differential obtained by the Abbott Alinity hq hematology analyzer to an 8-color single-tube flow cytometry method and to manual WBC differential.
Samples from 144 patients were tested with Alinity hq, flow cytometry, and microscopic WBC differential. The WBC count ranged from 1.22 to 359 × 10 /L, and 34 samples were flagged by the analyzer for abnormal WBC morphology.
Strong concordance was demonstrated between Alinity hq and flow cytometry for all six components of the differential, with correlation coefficients ranging from 0.86 (basophils) to 1.00 (lymphocytes). Small, clinically insignificant positive difference was observed between Alinity hq and flow cytometry for mature and total neutrophils (2.51% and 1.85%) and eosinophils (0.14%), and small negative difference for immature granulocytes (-0.65%), lymphocytes (-0.61%), and basophils (-0.21%). No bias was detected between the Alinity hq and flow cytometry monocyte counts. Alinity hq and flow cytometry results agreed with the manual differential, apart from small, clinically insignificant differences. Alinity hq nucleated red blood cell concentrations were equivalent with the manual results (r = 0.95, slope = 1.16). The percentage of blasts by flow cytometry demonstrated good correlation and agreement with the manual count (r = 0.99, slope = 1.35).
Alinity hq has produced accurate six-part WBC differential in this three-way comparison, equivalent to flow cytometry and morphological classification.
流式细胞术检测白细胞（WBC）分类可报告六分群 WBC 分类并计数原始细胞。一些现代血液分析仪还能够提供六分群 WBC 分类（包括不成熟粒细胞）。我们的目标是比较 Abbott Alinity hq 血液分析仪获得的 WBC 分类与 8 色单管流式细胞术方法和手工 WBC 分类的结果。
对 144 例患者的样本进行 Alinity hq、流式细胞术和显微镜下 WBC 分类检测。WBC 计数范围为 1.22 至 359×10/L，34 例样本的分析仪提示 WBC 形态异常。
Alinity hq 与流式细胞术在六分群的所有六个分类中均显示出较强的一致性，相关系数范围为 0.86（嗜碱性粒细胞）至 1.00（淋巴细胞）。Alinity hq 与流式细胞术在成熟和总中性粒细胞（2.51%和 1.85%）和嗜酸性粒细胞（0.14%）之间观察到微小的、临床无意义的正差异，不成熟粒细胞（-0.65%）、淋巴细胞（-0.61%）和嗜碱性粒细胞（-0.21%）之间观察到微小的负差异。在单核细胞计数方面，未检测到 Alinity hq 与流式细胞术之间存在偏差。除了微小的、临床无意义的差异外，Alinity hq 与手工分类结果一致。Alinity hq 有核红细胞浓度与手工结果相当（r=0.95，斜率=1.16）。流式细胞术检测原始细胞的百分比与手工计数具有良好的相关性和一致性（r=0.99，斜率=1.35）。
在这项三方比较中，Alinity hq 产生了准确的六分群 WBC 分类，与流式细胞术和形态学分类相当。
