Gardner P D, Heinemann S, Patrick J
Molecular Neurobiology Laboratory, Salk Institute, San Diego, CA 92138-9216.
Brain Res. 1987 Dec;427(1):69-76. doi: 10.1016/0169-328x(87)90046-5.
The muscle nicotinic acetylcholine receptor undergoes profound changes in abundance and distribution in response to innervation and denervation. As a start towards understanding the detailed mechanisms of acetylcholine receptor gene regulation, we have identified transcriptional regulatory regions of a receptor subunit gene. A region of genomic DNA at the 5' end of the mouse acetylcholine receptor gamma-subunit gene has been shown to promote transcription of the bacterial chloramphenicol acetyltransferase gene in a cell-type specific manner. In addition, the transcriptional activity of this fragment is developmentally regulated in mouse muscle C2C12 cells. We propose that this fragment contains transcriptional control elements of the mouse muscle acetylcholine receptor gamma-subunit gene.
肌肉烟碱型乙酰胆碱受体在神经支配和去神经支配的情况下，其丰度和分布会发生深刻变化。作为理解乙酰胆碱受体基因调控详细机制的第一步，我们已经鉴定出一个受体亚基基因的转录调控区域。小鼠乙酰胆碱受体γ亚基基因5'端的一段基因组DNA已被证明能以细胞类型特异性的方式促进细菌氯霉素乙酰转移酶基因的转录。此外，该片段的转录活性在小鼠肌肉C2C12细胞中受到发育调控。我们认为该片段包含小鼠肌肉乙酰胆碱受体γ亚基基因的转录控制元件。
