Emerg Infect Dis. 2021 Dec;27(12):3073-3081. doi: 10.3201/eid2712.211150.
Zika virus (ZIKV) is a member of the Flaviviridae family, which includes other clinically notable viruses such as the 4 dengue virus serotypes (DENV-1-4). Distinguishing DENVs from ZIKV using the established serologic assays widely used for monitoring DENV transmission is difficult because of antibody cross-reactivity between these closely related flaviviruses. We describe a modified and improved recombinant envelope domain III-based serologic assay for detecting ZIKV type-specific antibodies in regions with endemic DENV transmission. When the assay was used to measure ZIKV seroprevalence in 2017 among children 9-14 years of age living in a region of the Philippines with endemic DENV transmission, we observed a ZIKV seroprevalence of 18%. Investigators should consider using the ZIKV envelope domain III-based assay, which is simple and readily adaptable for use in standard clinical and public health laboratories, to assess ZIKV seroprevalence in areas with endemic DENV transmission.
Zika 病毒（ZIKV）是黄病毒科的一个成员，该科还包括其他临床上重要的病毒，如 4 种登革热病毒血清型（DENV-1-4）。由于这些密切相关的黄病毒之间存在抗体交叉反应，因此使用广泛用于监测 DENV 传播的既定血清学检测方法来区分 DENVs 和 ZIKV 很困难。我们描述了一种改良和改进的基于重组包膜域 III 的血清学检测方法，用于检测在登革热地方性传播地区的 Zika 病毒型特异性抗体。当该检测方法用于测量 2017 年在菲律宾一个登革热地方性传播地区居住的 9-14 岁儿童中的 Zika 病毒血清流行率时，我们观察到 18%的 Zika 病毒血清流行率。研究人员应考虑使用基于 Zika 病毒包膜域 III 的检测方法，该方法简单且易于适应在标准临床和公共卫生实验室中使用，以评估在登革热地方性传播地区的 Zika 病毒血清流行率。
