Duke University School of Medicine, USA.
Department of Neurobiology, USA.
Epilepsy Res. 2021 Dec;178:106816. doi: 10.1016/j.eplepsyres.2021.106816. Epub 2021 Nov 14.
We set out to establish a novel model of temporal lobe epilepsy (TLE) in a mouse. We sought to induce TLE through the injection of kainic acid (KA) into the tail vein with subsequent development of status epilepticus (SE). Using C57BL/6 mice, we implanted hippocampal EEG recording electrodes before or after injection of KA or phosphate buffered saline (PBS). Video and EEG analysis were conducted to evaluate for SE and development of recurrent seizures, the hallmark of TLE. All mice injected with KA developed SE while those who were injected with PBS did not. Of the animals injected with KA monitored for recurrent seizures following SE, 33% developed spontaneous recurrent seizures while those injected with PBS did not. Injection of KA through the tail vein of a mouse reliably and rapidly induces SE which remits spontaneously and leads to the development of TLE in a subset of mice.
我们着手建立一种新的颞叶癫痫（TLE）小鼠模型。我们试图通过尾静脉注射海人酸（KA）诱导 TLE，随后发展为癫痫持续状态（SE）。使用 C57BL/6 小鼠，我们在注射 KA 或磷酸盐缓冲盐水（PBS）之前或之后植入海马脑电记录电极。进行视频和 EEG 分析，以评估 SE 和复发性癫痫的发展，这是 TLE 的标志。所有注射 KA 的小鼠均发生 SE，而注射 PBS 的小鼠则未发生 SE。在 SE 后监测复发性癫痫的 KA 注射动物中，33%自发出现复发性癫痫，而注射 PBS 的动物则没有。通过尾静脉向小鼠注射 KA 可可靠且快速地诱导 SE，SE 会自发缓解，并导致一部分小鼠发展为 TLE。
