Opt Express. 2021 Oct 11;29(21):33679-33693. doi: 10.1364/OE.435783.
This study presents a partially coherent illumination based (PCI-based) SIM apparatus for dual-modality (phase and fluorescent) microscopic imaging. The partially coherent illumination (PCI) is generated by placing a rotating diffuser on a monochromatic laser beam, which suppresses speckle noise in the dual-modality images and endows the apparatus with sound sectioning capability. With this system, label-free quantitative phase and super-resolved/sectioned fluorescent images can be obtained for the same sample. We have demonstrated the superiority of the system in phase imaging of transparent cells with high endogenous contrast and in a quantitative manner. In the meantime, we have also demonstrated fluorescent imaging of fluorescent beads, rat tail crosscut, wheat anther, and hibiscus pollen with super-resolution and optical sectioning. We envisage that the proposed method can be applied to many fields, including but not limited to biomedical, industrial, chemistry fields.
本研究提出了一种基于部分相干照明（PCI）的 SIM 仪器，用于双模态（相和荧光）显微镜成像。部分相干照明（PCI）通过在单色激光束上放置旋转漫射器产生，可抑制双模态图像中的散斑噪声，并赋予仪器良好的切片能力。使用该系统，可以对同一样品进行无标记定量相位和超高分辨率/切片荧光成像。我们已经证明了该系统在具有高内源性对比度的透明细胞的相位成像中具有优越性，并且可以进行定量分析。同时，我们还展示了具有超分辨率和光学切片的荧光珠、大鼠尾横切、小麦花药和芙蓉花粉的荧光成像。我们预计，所提出的方法可以应用于许多领域，包括但不限于生物医学、工业、化学领域。
