Gao Zijun, Han Keyi, Hua Xuanwen, Liu Wenhao, Jia Shu
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Biomed Opt Express. 2024 May 3;15(6):3574-3585. doi: 10.1364/BOE.521521. eCollection 2024 Jun 1.
Super-resolution microscopy has emerged as an indispensable methodology for probing the intricacies of cellular biology. Structured illumination microscopy (SIM), in particular, offers an advantageous balance of spatial and temporal resolution, allowing for visualizing cellular processes with minimal disruption to biological specimens. However, the broader adoption of SIM remains hampered by the complexity of instrumentation and alignment. Here, we introduce speckle-illumination super-resolution microscopy using hydrogel diffusers (SIM). The study utilizes the high scattering and optical transmissive properties of hydrogel materials and realizes a remarkably simplified approach to plug-in super-resolution imaging via a common epi-fluorescence platform. We demonstrate the SIM system using various phantom and biological samples, and the results exhibited effective 3D resolution doubling, optical sectioning, and high contrast. We foresee SIM, a cost-effective, biocompatible, and user-accessible super-resolution methodology, to significantly advance a wide range of biomedical imaging and applications.
超分辨率显微镜已成为探究细胞生物学复杂性不可或缺的方法。特别是结构光照明显微镜（SIM），在空间和时间分辨率之间实现了有利的平衡，能够在对生物样本干扰最小的情况下可视化细胞过程。然而，SIM的更广泛应用仍然受到仪器设备复杂性和校准的阻碍。在此，我们介绍使用水凝胶扩散器的散斑照明超分辨率显微镜（SIM）。该研究利用水凝胶材料的高散射和光学透射特性，通过常见的落射荧光平台实现了一种显著简化的插入式超分辨率成像方法。我们使用各种模型和生物样本演示了该SIM系统，结果显示出有效的三维分辨率加倍、光学切片和高对比度。我们预计，SIM作为一种经济高效、生物相容性好且用户可及的超分辨率方法，将显著推动广泛的生物医学成像及应用发展。
