von Meyenburg K, Nielsen L D, Johnsen K, Molin S, Svenningsen B, Miozzari G
Antimicrob Agents Chemother. 1978 Feb;13(2):234-43. doi: 10.1128/AAC.13.2.234.
Growth of the permeable strain AS19 of Escherichia coli B is more sensitive to the antibiotic streptolydigin than is in vitro ribonucleic acid (RNA) synthesis. The in vivo chain elongation rates of lacZ messenger RNA and ribosomal RNA are not affected at 1.5 x 10(-6) M, a concentration that reduces the growth rate threefold. The synthesis of large proteins is inhibited preferentially, and a considerable fraction of the polypeptides synthesized is unstable. The synthesis of complete beta-galactosidase is inhibited relative to the synthesis of short, unstable polypeptides, which include the first 60 to 70 amino acids of beta-galactosidase. The expression of the following polycistronic transcription units is strongly biased against promoter-distal genes: trp, deo, rpoBC, and rrn. The extent of polarity is proportional to the distance transcribed and to the streptolydigin concentration. Streptolydigin appears to destabilize active transcription complexes irreversibly irrespective of the type of transcript (messenger RNA, ribosomal RNA) and of transcription intensity. We suggest that streptolydigin leads to premature termination of transcription, resulting in release of incomplete transcripts and, thus, a decrease in overall messenger RNA concentration, which becomes limiting for protein synthesis, i.e., for growth.
大肠杆菌B的可渗透菌株AS19的生长比体外核糖核酸（RNA）合成对抗生素链霉溶菌素更为敏感。在1.5×10⁻⁶ M的浓度下，乳糖操纵子信使RNA和核糖体RNA的体内链延伸速率不受影响，而该浓度会使生长速率降低三倍。大蛋白质的合成优先受到抑制，并且合成的相当一部分多肽不稳定。完整的β-半乳糖苷酶的合成相对于短的、不稳定的多肽（包括β-半乳糖苷酶的前60至70个氨基酸）的合成受到抑制。以下多顺反子转录单位的表达强烈偏向于启动子远端基因：色氨酸操纵子、脱氧核糖核酸操纵子、RNA聚合酶β'和β亚基操纵子以及核糖体RNA操纵子。极性程度与转录距离和链霉溶菌素浓度成正比。无论转录物类型（信使RNA、核糖体RNA）和转录强度如何，链霉溶菌素似乎都会不可逆地破坏活性转录复合物的稳定性。我们认为链霉溶菌素导致转录过早终止，从而导致不完整转录物的释放，进而导致整体信使RNA浓度降低，这对蛋白质合成即生长产生限制作用。
