Bleyman M, Kondo M, Hecht N, Woese C
J Bacteriol. 1969 Aug;99(2):535-43. doi: 10.1128/jb.99.2.535-543.1969.
The transcriptional mapping method developed previously has been used to investigate the functional organization of the ribosomal ribonucleic acid (rRNA) and transfer ribonucleic acid (tRNA) cistrons in the Bacillus subtilis genome. The sensitivities of the syntheses of these RNA species to actinomycin D indicated that (i) large polycistronic groupings of the tRNA cistrons exist, but (ii) the 16S and 23S rRNA cistrons are probably transcribed as individual units, and (iii) each 5S rRNA cistron is part of an operon containing a 23S rRNA cistron.
先前开发的转录图谱绘制方法已用于研究枯草芽孢杆菌基因组中核糖体核糖核酸(rRNA)和转移核糖核酸(tRNA)顺反子的功能组织。这些RNA种类的合成对放线菌素D的敏感性表明:(i)tRNA顺反子存在大的多顺反子分组,但(ii)16S和23S rRNA顺反子可能作为单个单元转录,并且(iii)每个5S rRNA顺反子是包含一个23S rRNA顺反子的操纵子的一部分。