绿茶儿茶素对变形链球菌的抗菌活性-一项研究。

Green tea catechins showed antibacterial activity on streptococcus mutans -An study.

机构信息

Department of Periodontology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.

Department of Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.

出版信息

Indian J Dent Res. 2021 Apr-Jun;32(2):226-229. doi: 10.4103/ijdr.ijdr_512_21.

DOI:10.4103/ijdr.ijdr_512_21

PMID:34810394

Abstract

BACKGROUND

The main bacterial aetiological agents in caries formation are the α-haemolytic Streptococcal species Streptococcus mutans, which has been found to be the initiator of most dental caries. The leaves of Camellia sinensis known as green tea, has properties, such as antibacterial and anti-cariogenic. Epigallocatechin-3-gallate (EGCG) one of the most abundant catechins found in green tea is known to contribute to these effects.

AIM

To evaluate the antibacterial effect of green tea catechins namely EGCG on S. mutans with two different methods at different concentrations.

OBJECTIVES

To assess the antimicrobial efficacy of EGCG by disc diffusion test at concentrations of 100, 75, and 50 μg/mL. 2) To assess the antimicrobial efficacy of EGCG by Minimum inhibitory concentration (MIC) test at concentrations ranging from 0.2 to 100 μg/mL.

METHODOLOGY

Commercially available purest form of green tea polyphenol EGCG was used in the study. Disc diffusion test on agar medium and MIC test was used to determine the susceptibility of the S. mutans to green tea catechins EGCG.

RESULTS

The results of the agar well diffusion method showed that the EGCG extract has shown zones of inhibition against S. mutans at concentrations of 100 μg/mL (28.67 mm), 75 μg/mL (15.33 mm), 50 μg/mL (10.33 mm) while that of MIC test of EGCG extract of concentrations ranging from 0.2 to 100 μg/mL against S. mutans shows that the mean MIC value was 1.07.

CONCLUSION

Catechins in the tea are potentially anti-cariogenic agents which can reduce bacterial presence in the oral cavity and have the potential to be further used for the preparation of dentifrice and mouthwash.

摘要

背景

龋齿形成的主要细菌病原体是α-溶血性链球菌属的变形链球菌，它被认为是大多数龋齿的始动因子。茶叶的叶子，即绿茶，具有抗菌和抗龋的特性。表没食子儿茶素没食子酸酯（EGCG）是绿茶中含量最丰富的儿茶素之一，已知它对这些作用有贡献。

目的

用两种不同的方法，在不同的浓度下，评估绿茶儿茶素即 EGCG 对变形链球菌的抗菌作用。

目标

1）通过 100、75 和 50 μg/mL 的纸片扩散试验评估 EGCG 的抗菌功效。2）通过 0.2 至 100 μg/mL 浓度范围的最小抑菌浓度（MIC）试验评估 EGCG 的抗菌功效。

方法

研究中使用了市售的最纯形式的绿茶多酚 EGCG。在琼脂培养基上进行纸片扩散试验和 MIC 试验，以确定 S. mutans 对绿茶儿茶素 EGCG 的敏感性。

结果

琼脂孔扩散法的结果表明，EGCG 提取物在 100 μg/mL（28.67 mm）、75 μg/mL（15.33 mm）、50 μg/mL（10.33 mm）浓度下对 S. mutans 显示出抑制区，而 MIC 试验的 EGCG 提取物浓度范围为 0.2 至 100 μg/mL 对 S. mutans 显示平均 MIC 值为 1.07。