前列腺素内过氧化物21. 左型前列腺素E2与蛋白质的共价结合。
Prostaglandin endoperoxides 21. Covalent binding of levuglandin E2 with proteins.
作者信息
Salomon R G, Jirousek M R, Ghosh S, Sharma R B
机构信息
Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-2699.
出版信息
Prostaglandins. 1987 Nov;34(5):643-56. doi: 10.1016/0090-6980(87)90289-9.
Levuglandin E2 (LGE2), a gamma-ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, binds covalently with ram seminal vesicle microsomes. Totally synthetic 5,6-ditritio-LGE2 was prepared and used to determine that rapid covalent binding of LGE2 (initially 800 microM) occurs with 6.4 microM bovine serum albumin (greater than 10 equiv within 1 min) which approaches saturation (approximately 16 equiv) after 40 min at 37 degrees C.
列伏前列素E2（LGE2）是在其生物合成的水性条件下由前列腺素内过氧化物PGH2重排产生的γ-酮醛，它与公羊精囊微粒体共价结合。制备了全合成的5,6-二氚代-LGE2，并用于确定LGE2（初始浓度为800微摩尔）与6.4微摩尔牛血清白蛋白迅速发生共价结合（1分钟内大于10当量），在37℃下40分钟后接近饱和（约16当量）。
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