Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia Farroupilha, Frederico Westphalen, Brazil.
J Appl Microbiol. 2022 Mar;132(3):2067-2079. doi: 10.1111/jam.15363. Epub 2021 Nov 29.
To investigate the potential of novel Bacillus velezensis P45 as an eco-friendly alternative for bioprocessing poultry by-products into valuable antimicrobial products.
The complete genome of B. velezensis P45 was sequenced using the Illumina MiSeq platform, showing 4455 protein and 98 RNA coding sequences according to the annotation on the RAST server. Moreover, the genome contains eight gene clusters for the production of antimicrobial secondary metabolites and 25 putative protease-related genes, which can be related to feather-degrading activity. Then, in vitro tests were performed to determine the production of antimicrobial compounds using feather, feather meal and brain-heart infusion (BHI) cultures. Antimicrobial activity was observed in feather meal and BHI media, reaching 800 and 3200 AU ml against Listeria monocytogenes respectively. Mass spectrometry analysis indicates the production of antimicrobial lipopeptides surfactin, fengycin and iturin.
The biotechnological potential of B. velezensis P45 was deciphered through genome analysis and in vitro studies. This strain produced antimicrobial lipopeptides growing on feather meal, a low-cost substrate.
The production of antimicrobial peptides by this keratinolytic strain may represent a sustainable alternative for recycling by-products from poultry industry. Furthermore, whole B. velezensis P45 genome sequence was obtained and deposited.
研究新型芽孢杆菌 P45 作为一种环保替代品的潜力，将家禽副产物生物加工成有价值的抗菌产品。
使用 Illumina MiSeq 平台对芽孢杆菌 P45 的全基因组进行测序，根据 RAST 服务器的注释显示，共有 4455 个蛋白和 98 个 RNA 编码序列。此外，基因组包含 8 个用于产生抗菌次生代谢物的基因簇和 25 个假定的与蛋白酶相关的基因，这些基因可能与羽毛降解活性有关。然后，进行了体外试验以确定使用羽毛、羽毛粉和脑心浸液（BHI）培养物产生抗菌化合物。在羽毛粉和 BHI 培养基中观察到抗菌活性，分别对单核细胞增生李斯特菌达到 800 和 3200 AU ml。质谱分析表明产生了抗菌脂肽表面活性剂、fengycin 和iturin。
通过基因组分析和体外研究揭示了芽孢杆菌 P45 的生物技术潜力。该菌株在羽毛粉等低成本基质上生长时产生了抗菌脂肽。
该角蛋白分解菌株产生抗菌肽可能代表了家禽工业副产物回收的可持续替代方案。此外，还获得并提交了完整的芽孢杆菌 P45 基因组序列。
